Monitoring demyelination and remyelination by magnetization transfer imaging in the mouse brain at 9.4 T.
Monitoring demyelination and remyelination by magnetization transfer imaging in the mouse brain at 9.4 T.
复制标题
通过 9.4 T 的磁化转移成像监测小鼠大脑中的脱髓鞘和髓鞘再生。
DOI：
10.1007/s10334-008-0141-3
复制
发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Dousset,Vincent
中科院分区：
文献类型：
--
作者：
Zaaraoui,Wafaa;Deloire,Mathilde;Merle,Michel;Girard,Céline;Raffard,Gérard;Biran,Marc;Inglese,Matilde;Petry,KlausG;Gonen,Oded;Brochet,Bruno;Franconi,Jean-Michel;Dousset,Vincent
本研究的目的是通过磁化传递成像（MTI）定量评估脱髓鞘和髓鞘再生过程中髓鞘含量的结构变化。材料和方法在一个可逆的脱髓鞘模型中，在9.4T下用MTI研究了铜腙中毒的小鼠体内无轴突损失。结果磁共振成像（MRI）结果显示，在脱髓鞘过程中，磁化传递率（MTR）显著降低，在髓鞘再生过程中，MTR显著升高，且与髓鞘含量呈显著相关（r= 0. 79，P = 0. 01）。这可能导致在体内监测促进髓鞘再生的治疗策略。
ObjectiveThe aim of this study was to assess quantitatively structural changes in myelin content occurring during demyelination and remyelination by magnetization transfer imaging (MTI).Materials and methodsIn a reversible model of demyelination with no axonal loss, mice intoxicated by cuprizone were studied by MTI in vivo at 9.4 T. MRI data were compared to histopathological examinations.ResultsData revealed that the magnetization transfer ratio (MTR) decreased significantly during demyelination and increased during remyelination with strong correlation to the myelin content (r= 0.79,P= 0.01).ConclusionsThis study demonstrated that MTR is a sensitive and reproducible quantitative marker to assess myelin loss and repair. This may lead to in vivo monitoring of therapeutic strategies promoting remyelination.