Process performance and comparative metagenomic analysis during co-digestion of manure and lignocellulosic biomass for biogas production
Process performance and comparative metagenomic analysis during co-digestion of manure and lignocellulosic biomass for biogas production
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DOI：
10.1016/j.apenergy.2016.10.081
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发表时间：
2017
期刊：
影响因子：
11.2
通讯作者：
Panagiotis Tsapekos;P. Kougias;L. Treu;S. Campanaro;I. Angelidaki
中科院分区：
文献类型：
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作者：
Panagiotis Tsapekos;P. Kougias;L. Treu;S. Campanaro;I. Angelidaki
机械预处理被认为是制备厌氧消化用生物质的一种快速、简便的方法。在本研究中，机械预处理对木质纤维素青贮饲料的生物降解性的影响在间歇反应器中阐明。此外，共消化的青贮饲料与猪粪连续进料沼气反应器进行了研究。通过分析未组装的鸟枪基因组序列进行宏基因组分析，以确定在猪粪消化系统中的微生物群落。比较分析允许识别牢固附着在消化的草颗粒上的微生物物种，并将它们与漂浮在液体介质中的浮游微生物区分开。结果表明，机械预处理能显著提高青贮牧草的甲烷产量，提高幅度为12.3%。同样，在共消化系统中的甲烷产率的增加达到6.4%。关于宏基因组学研究，发现分别以高蛋白水解和纤维素分解活性而闻名的粪热杆菌和热纤梭菌类似的物种牢固地附着在消化原料的固体部分上。液体样品的结果显示，微生物群落组成的明显差异，主要由变形菌。在共消化实验的液体部分中，古菌群落的相对丰度高于固体部分。最后，一个未分类的Alkaliphilussp.在所有样品中发现了高相对丰度。
Mechanical pretreatment is considered to be a fast and easily applicable method to prepare the biomass for anaerobic digestion. In the present study, the effect of mechanical pretreatment on lignocellulosic silages biodegradability was elucidated in batch reactors. Moreover, co-digestion of the silages with pig manure in continuously fed biogas reactors was examined. Metagenomic analysis for determining the microbial communities in the pig manure digestion system was performed by analysing unassembled shotgun genomic sequences. A comparative analysis allowed to identify the microbial species firmly attached to the digested grass particles and to distinguish them from the planktonic microbes floating in the liquid medium. It was shown that the methane yield of ensiled grass was significantly increased by 12.3% due to mechanical pretreatment in batch experiments. Similarly, the increment of the methane yield in the co-digestion system reached 6.4%. Regarding the metagenomic study, species similar toCoprothermobacter proteolyticusand to Clostridium thermocellum, known for high proteolytic and cellulolytic activity respectively, were found firmly attached to the solid fraction of digested feedstock. Results from liquid samples revealed clear differences in microbial community composition, mainly dominated byProteobacteria. The archaeal community was found in higher relative abundance in the liquid fraction of co-digestion experiment compared to the solid fraction. Finally, an unclassifiedAlkaliphilussp. was found in high relative abundance in all samples.