SpectraCam® : A new polarized hyperspectral imaging system for repeatable and reproducible in vivo skin quantification of melanin, total hemoglobin, and oxygen saturation
SpectraCam® : A new polarized hyperspectral imaging system for repeatable and reproducible in vivo skin quantification of melanin, total hemoglobin, and oxygen saturation
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DOI：
10.1111/srt.12396
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发表时间：
2018-02-01
影响因子：
2.2
通讯作者：
Vie, K.
中科院分区：
文献类型：
--
作者：
Nkengne, A.;Robic, J.;Vie, K.
背景资料：确定皮肤色素沉着的准确方法是获取皮肤样品的光谱反射率，并通过光传播的物理模型的反向计算来量化发色团。因此，我们测试了一种新的高光谱成像设备和软件套件，SpectraCam（R）系统，并评估其准确性，以量化皮肤chromophores.Methods：验证的SpectraCam（R）系统进行，首先，比较已知的和获得的反射光谱的颜色幻影。然后由两名操作员评价重复性和再现性，他们在不同时间点进行采集并比较采集的反射光谱。通过对单个发色团变异模型（雀斑样痣和压力释放）的定量分析来测试该系统的特异性。最后，我们测试了SpectraCam（R）系统检测由于日常使用一种新的抗黑眼圈化妆品而引起的眼部发色团变化的能力。结果：SpectraCam（R）系统忠实地获取了彩色幻影的反射光谱（r（2）>0.90）。由不同操作者在不同时间获得的皮肤反射光谱具有高度的可重复性（r（2）>0.94）和可再现性（r（2）>0.99）。SpectraCam（R）系统还可以生成定性图，揭示皮肤发色团或血管等底层结构的局部变化。该系统足够精确，可以检测雀斑中的黑色素变化或总血红蛋白以及压力释放后的氧饱和度变化。由于使用了抗黑眼圈化妆品，该系统还能灵敏地检测眼部黑色素的减少。结论：SpectraCam（R）系统被证明是快速的，并能产生包含大视场的高分辨率数据。它是一个强大的高光谱成像系统，可以量化黑色素，总血红蛋白和氧饱和度，非常适合美容研究。
Background: An accurate way to determine skin pigmentation is to acquire the spectral reflectance of a skin sample and to quantify chromophores by reverse calculation from physical models of light propagation. Therefore, we tested a new hyperspectral imaging device and software suite, the SpectraCam (R) system, and evaluated its accuracy to quantify skin chromophores.Methods: Validation of the SpectraCam (R) system was performed by, firstly, comparing the known and the acquired reflectance spectra of color phantoms. Repeatability and reproducibility were then evaluated by two operators who performed acquisitions at different time points and compared the acquired reflectance spectra. The specificity of the system was tested by quantitative analysis of single chromophore variation models: lentigo and pressure relief. Finally, we tested the ability of the SpectraCam (R) system to detect variations in chromophore in the eye region due to the daily application of a new anti-dark circle cosmetic product.Results: The SpectraCam (R) system faithfully acquires the reflectance spectra of color phantoms (r(2)>0.90). The skin reflectance spectra acquired by different operators at different times are highly repeatable (r(2)>0.94) and reproducible (r(2)>0.99). The SpectraCam (R) system can also produce qualitative maps that reveal local variations in skin chromophore or underlying structures such as blood vessels. The system is precise enough to detect melanin variation in lentigo or total hemoglobin and oxygen saturation variations upon pressure relief. It is also sensitive enough to detect a decrease in melanin in the eye region due to the application of an anti-dark circle cosmetic product.Conclusion: The SpectraCam (R) system proves to be rapid and produces high-resolution data encompassing a large field of view. It is a robust hyperspectral imaging system that quantifies melanin, total hemoglobin, and oxygen saturation and is well adapted to cosmetic research.