Hedgehog signaling - A biophysical or biomechanical modulator in embryonic development?
Hedgehog signaling - A biophysical or biomechanical modulator in embryonic development?
复制标题
DOI：
10.1196/annals.1389.029
复制
发表时间：
2007-01-01
期刊：
影响因子：
--
通讯作者：
Yamagishi, Masaaki
中科院分区：
文献类型：
--
作者：
Nagase, Takashi;Nagase, Miki;Yamagishi, Masaaki
尽管胚胎发育不可避免地受到生物物理或生物力学过程的影响，但尚未阐明发育的分子机制在多大程度上受到这些物理因素的调节。刺猬家族，包括声波刺猬（Shh），是最著名的形态发生素，参与神经系统、面部、四肢和皮肤附属器等各种器官的发育模式形成。刺猬信号传导有几个独特的特征，包括长程扩散或正负反馈回路，表明生物物理或生物力学因素可能对刺猬信号传导进行修改。特别是，胚胎第8-10天的时期以小鼠发育中的各种生物力学调节过程为特征，例如轴旋转和血管生成。我们使用小鼠全胚胎培养系统进行了一系列实验，以研究刺猬信号在此期间的生物力学作用。在这篇综述中，我们研究了揭示刺猬信号在发育过程中的生物物理和生物力学方面的各种例子，包括我们自己使用小鼠全胚胎培养系统的数据。
Although embryonic development is inevitably affected by biophysical or biomechanical processes, it has yet to be elucidated to what extent molecular mechanisms of development are modulated by such physical factors. The hedgehog family, including Sonic hedgehog (Shh), is the most well-known morphogens involved in the developmental pattern formation of various organs, such as the nervous system, face, limbs, and skin appendages. There are several unique features in hedgehog signaling including long-range diffusion or positive and negative feedback loops, suggesting the possible modification of hedgehog signaling by biophysical or biomechanical factors. Especially, the period of embryonic day 8-10 is characterized by various biomechanically regulated processes in mouse development, such as axial rotation and vasculoangiogenesis. We executed a series of experiments using a mouse whole embryo culture system to investigate the biomechanical roles of hedgehog signaling during this period. In this review, we examine various examples in which biophysical and biomechanical aspects of hedgehog signaling in development are revealed, including our own data using the mouse whole embryo culture system.