Environmental enrichment brings a beneficial effect on beam walking and enhances the migration of doublecortin-positive cells following striatal lesions in rats
Environmental enrichment brings a beneficial effect on beam walking and enhances the migration of doublecortin-positive cells following striatal lesions in rats
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DOI：
10.1016/j.neuroscience.2006.10.038
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发表时间：
2007-02-09
期刊：
影响因子：
3.3
通讯作者：
Nishino, H.
中科院分区：
文献类型：
--
作者：
Urakawa, S.;Hida, H.;Nishino, H.
据报道，在富集环境中饲养的大鼠（富集大鼠）在脑损伤后表现出较少的运动功能障碍，但这种改善的神经元相关性尚未得到很好的阐明。本研究旨在阐明化学脑损伤和环境富集对运动功能和损伤诱导的神经发生的影响。三周龄、刚断奶的大鼠分为两组：一组在强化环境中饲养，另一组在标准笼中饲养5周。8周龄时，将神经毒素6-羟基多巴胺（6-OHDA）或喹啉酸（QA）注射到纹状体中，或将6-OHDA注射到黑质（SN）中，使黑质纹状体多巴胺能神经支配减少，从而诱导纹状体损伤。与标准条件下饲养的大鼠相比，营养丰富的大鼠在梁行走中表现出更好的表现，但在圆柱体测试中，两组大鼠都表现出类似的前肢使用不对称性。脑室下区溴脱氧尿嘧啶标记的增殖细胞数量在严重纹状体病变后1周增加，但在SN注射6-OHDA后减少。纹状体注射6-OHDA或CIA诱导病变后，纹状体中cloublecortin （DCX）阳性细胞数量显著增加；然而，6-OHDA注射到SN后，dcx阳性细胞的数量没有变化。环境富集使背纹状体中dcx阳性细胞呈迁移形态增加。在富集的大鼠中，dcx阳性细胞穿过远离胼胝体和侧脑室的纹状体实质。dcx阳性细胞共表达未成熟神经元标记物，多唾液化神经细胞粘附分子，但胶质标记物阴性。这些数据表明，环境富集改善了梁行走的运动表现，并增强了神经元向纹状体损伤区域的迁移。(c) 2006 IBRO。Elsevier Ltd.出版。版权所有。
Rats raised in an enriched environment (enriched rats) have been reported to show less motor dysfunction following brain lesions, but the neuronal correlates of this improvement have not been well clarified. The present study aimed to elucidate the effect of chemical brain lesions and environmental enrichment on motor function and lesion-induced neurogenesis. Three week-old, recently weaned rats were divided into two groups: one group was raised in an enriched environment and the other group was raised in a standard cage for 5 weeks. Striatal damage was induced at an age of 8 weeks by injection of the neuro-toxins 6-hydroxydoparnine (6-OHDA) or quinolinic acid (QA) into the striaturn, or by injection of 6-OHDA into the substantia nigra (SN), which depleted nigrostriatal doparninergic innervation. Enriched rats showed better performance on beam walking compared with those raised in standard conditions, but both groups showed similar forelimb use asymmetry in a cylinder test. The number of bromodeoxyuridine-labeled proliferating cells in the subventricular zone was increased by a severe striatal lesion induced by QA injection 1 week after the lesion, but decreased by injection of 6-OHDA into the SN. Following induction of lesions by striatal injection of 6-OHDA or CIA, the number of cells positive for cloublecortin (DCX) was strongly increased in the striatum; however, there was no change in the number of DCX-positive cells following 6-OHDA injection into the SN. Environmental enrichment enhanced the increase of DCX-positive cells with migrating morphology in the dorsal striaturn. In enriched rats, DCX-positive cells traversed the striatal parenchyma far from the corpus callosurn and lateral ventricle. DCX-positive cells co-expressed an immature neuronal marker, polysialylated neural cell adhesion molecule, but were negative for a glial marker. These data suggest that environmental enrichment improves motor performance on beam walking and enhances neuronal migration toward a lesion area in the striaturn. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.