The failure to express a protein disulphide isomerase-like protein results in a floury endosperm and an endoplasmic reticulum stress response in rice.
The failure to express a protein disulphide isomerase-like protein results in a floury endosperm and an endoplasmic reticulum stress response in rice.
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未能表达二硫化物异构酶样蛋白会导致水稻粉质胚乳和内质网应激反应
DOI：
10.1093/jxb/err262
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发表时间：
2012-01
影响因子：
6.9
通讯作者：
Wan J
中科院分区：
文献类型：
--
作者：
Han X;Wang Y;Liu X;Jiang L;Ren Y;Liu F;Peng C;Li J;Jin X;Wu F;Wang J;Guo X;Zhang X;Cheng Z;Wan J
水稻体细胞无性系突变体T3612产生的小粒具有粉状胚乳，这是由于淀粉粒的松散堆积造成的。突变的定位克隆显示编码蛋白二硫化物异构酶样酶(PDIL1-1)的基因缺失。在野生型中，PDIL1-1在整个植株中都有表达，但在发育中的籽粒中表达最强。在T3612中，其表达被取消，导致胞质磷酸化酶和普鲁兰酶活性降低，而可溶性淀粉合成酶I和ADP-葡萄糖焦磷酸化酶活性增加。与野生型相比，T3612胚乳中支链淀粉的聚合度为8-13。突变体胚乳中某些内质网胁迫反应基因的表达显著增强。PDIL1-1似乎在淀粉合成中起着重要作用。它的缺失与胚乳中的内质网胁迫有关，这可能是T3612突变体形成粉状胚乳的基础。
The rice somaclonal mutant T3612 produces small grains with a floury endosperm, caused by the loose packing of starch granules. The positional cloning of the mutation revealed a deletion in a gene encoding a protein disulphide isomerase-like enzyme (PDIL1-1). In the wild type, PDIL1-1 was expressed throughout the plant, but most intensely in the developing grain. In T3612, its expression was abolished, resulting in a decrease in the activity of plastidial phosphorylase and pullulanase, and an increase in that of soluble starch synthase I and ADP-glucose pyrophosphorylase. The amylopectin in the T3612 endosperm showed an increase in chains with a degree of polymerization 8–13 compared with the wild type. The expression in the mutant's endosperm of certain endoplasmic reticulum stress-responsive genes was noticeably elevated. PDIL1-1 appears to play an important role in starch synthesis. Its absence is associated with endoplasmic reticulum stress in the endosperm, which is likely to underlie the formation of the floury endosperm in the T3612 mutant.
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影响因子：
7.4
作者：
Hennen-Bierwagen, Tracie A.;Lin, Qiaohui;Myers, Alan M.
通讯作者：
Myers, Alan M.
影响因子：
7.4
作者：
Colleoni, C;Dauvillée, D;Ball, S
通讯作者：
Ball, S
影响因子：
6.9
作者：
Fujita N;Toyosawa Y;Utsumi Y;Higuchi T;Hanashiro I;Ikegami A;Akuzawa S;Yoshida M;Mori A;Inomata K;Itoh R;Miyao A;Hirochika H;Satoh H;Nakamura Y
通讯作者：
Nakamura Y
影响因子：
7.4
作者：
Hennen-Bierwagen, Tracie A.;Liu, Fushan;Myers, Alan M.
通讯作者：
Myers, Alan M.
影响因子：
7.4
作者：
Kubo, A;Fujita, N;Nakamura, Y
通讯作者：
Nakamura, Y