AtPep3 is a hormone-like peptide that plays a role in the salinity stress tolerance of plants
AtPep3 is a hormone-like peptide that plays a role in the salinity stress tolerance of plants
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DOI：
10.1073/pnas.1719491115
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发表时间：
2018-05-29
影响因子：
11.1
通讯作者：
Hanada, Kousuke
中科院分区：
文献类型：
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作者：
Nakaminami, Kentaro;Okamoto, Masanori;Hanada, Kousuke
由小的编码基因编码的肽在植物发育中起着重要作用，以类似于植物激素的方式起作用。然而，很少有类大豆肽在非生物胁迫耐受性中发挥作用。在本研究中，发现17个编码小肽的拟南芥基因在盐胁迫下表达上调。为了鉴定导致盐胁迫耐受性的肽，我们产生了过表达这些小编码基因的转基因拟南芥植物，并评估了盐胁迫条件下的存活率和根生长。结果表明，17个过表达基因中有4个提高了耐盐性。进一步的研究集中在AtPROPEP 3，这是在盐胁迫下表达最高的基因。用AtPROPEP 3编码的合成肽处理植物，发现C-末端肽片段（AtPep 3）抑制盐诱导的幼苗叶绿素漂白。相反，敲低AtPROPEP 3转基因植物在盐胁迫下表现出超敏表型，这是由AtPep 3肽补充。该功能性AtPep 3肽区域与与植物免疫应答相关的AtPep 3激发子肽重叠。AtPep 3受体突变体的功能分析表明，AtPep 3被PEPR 1受体识别，它的功能是增加植物的耐盐胁迫性。总的来说，这些数据表明AtPep 3在拟南芥的盐胁迫耐受性和免疫应答中起着重要作用。
Peptides encoded by small coding genes play an important role in plant development, acting in a similar manner as phytohormones. Few hormone-like peptides, however, have been shown to play a role in abiotic stress tolerance. In the current study, 17 Arabidopsis genes coding for small peptides were found to be up-regulated in response to salinity stress. To identify peptides leading salinity stress tolerance, we generated transgenic Arabidopsis plants over-expressing these small coding genes and assessed survivability and root growth under salinity stress conditions. Results indicated that 4 of the 17 overexpressed genes increased salinity stress tolerance. Further studies focused on AtPROPEP3, which was the most highly up-regulated gene under salinity stress. Treatment of plants with synthetic peptides encoded by AtPROPEP3 revealed that a C-terminal peptide fragment (AtPep3) inhibited the salt-induced bleaching of chlorophyll in seedlings. Conversely, knockdown AtPROPEP3 transgenic plants exhibited a hypersensitive phenotype under salinity stress, which was complemented by the AtPep3 peptide. This functional AtPep3 peptide region overlaps with an AtPep3 elicitor peptide that is related to the immune response of plants. Functional analyses with a receptor mutant of AtPep3 revealed that AtPep3 was recognized by the PEPR1 receptor and that it functions to increase salinity stress tolerance in plants. Collectively, these data indicate that AtPep3 plays a significant role in both salinity stress tolerance and immune response in Arabidopsis.