INFLUENZA-A VIRUS NUCLEOPROTEIN IS A MAJOR TARGET ANTIGEN FOR CROSS-REACTIVE ANTI-INFLUENZA-A VIRUS CYTO-TOXIC LYMPHOCYTES-T
INFLUENZA-A VIRUS NUCLEOPROTEIN IS A MAJOR TARGET ANTIGEN FOR CROSS-REACTIVE ANTI-INFLUENZA-A VIRUS CYTO-TOXIC LYMPHOCYTES-T
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DOI：
10.1073/pnas.82.6.1785
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
MOSS, B
中科院分区：
文献类型：
--
作者：
YEWDELL, JW;BENNINK, JR;MOSS, B
甲型流感病毒特异性细胞毒性T淋巴细胞(CTL)能够裂解感染任何甲型流感病毒的细胞(交叉反应性CTL)，是宿主对流感的CTL反应的主要部分。病毒核蛋白(NP)是体内主要的病毒粒子结构蛋白，可能是交叉反应CTL的靶抗原。为了直接检测CTL对NP的识别作用，构建了含有甲型流感病毒NP基因DNA拷贝的痘苗病毒重组体。感染该病毒的小鼠细胞以主要组织相容性复合体受限的方式被多种甲型流感病毒免疫获得的交叉反应CTL群体有效地裂解。含有PR8 NP基因的重组痘苗病毒能够刺激和启动强烈的二次交叉反应CTL反应。用含PR8NP基因的重组痘苗病毒免疫小鼠的脾细胞能被3种主要亚型的甲型流感病毒刺激。最后，未标记的靶竞争实验表明，NP是交叉反应CTL识别的主要但不是唯一的病毒靶抗原。
Influenza A virus-specific cytotoxic T lymphocytes (CTL) capable of lysing cells infected with any influenza A virus (cross-reactive CTL) constitute a major portion of the host CTL response to influenza. The viral nucleoprotein (NP), a major internal virion structural protein, was implicated as a possible target antigen for cross-reactive CTL. To directly examine CTL recognition of NP, a vaccinia virus recombinant containing a DNA copy of an influenza A virus NP gene was constructed. Murine cells infected with this virus were efficiently lysed in a major histocompatibility complex-restricted manner by cross-reactive CTL populations obtained by immunization with a variety of influenza A virus subtypes. The recombinant vaccinia virus containing the PR8 NP gene was able to both stimulate and prime for a vigorous secondary cross-reactive CTL response. Splenocytes from mice primed by inoculation with the recombinant vaccinia virus containing the PR8 NP gene could be stimulated by influenza A viruses of all 3 major human subtypes. Finally, unlabeled target competition experiments suggest that NP is a major, but not the sole, viral target antigen recognized by cross-reactive CTL.