Oxidative injury and survival during endotoxemia
Oxidative injury and survival during endotoxemia
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DOI：
10.1016/s0014-5793(98)01290-3
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发表时间：
1998-11-06
期刊：
影响因子：
3.5
通讯作者：
Eriksson, M
中科院分区：
文献类型：
--
作者：
Basu, S;Eriksson, M
本研究在实验性猪内毒素性休克模型中研究了8-iso-PGF（2 α）（一种非酶促）和15-K-DH-PGF（2 α）（一种环氧合酶催化的花生四烯酸氧化产物）的血浆水平。内毒素输注后PGF（2 α）代谢物的显著（P < 0.001）和快速出现和消失在非存活组和存活组中非常相似，表明炎症的急性进展和消退。当通过测量游离8-iso-PGF（2 α）来评估氧化损伤时，血浆中的水平显著增加至2小时，并且在非存活者中保持在该水平直至死亡。这与存活者的8-iso-PGF（2 α）水平在1h时升高至高峰，然后在5 h后降至基础水平明显不同。因此，在内毒素血症期间发生自由基和环氧合酶催化的花生四烯酸氧化，内毒素诱导的炎症后的自由基依赖性氧化损伤可能是器官衰竭和死亡率增加的主要原因。(C)1998年欧洲生物化学学会联合会。
This study investigates the plasma levels of 8-iso-PGF(2 alpha), a non-enzymatic, and 15-K-DH-PGF(2 alpha), a cyclooxygenase catalyzed oxidation product of arachidonic acid in an experimental porcine endotoxemic shock model. A significant (P < 0.001) and rapid appearance and disappearance of PGF(2 alpha) metabolite after endotoxin infusion was very similar in both non-survival and survival groups indicating an acute progression and recession of inflammation. When oxidative injury was assessed by measuring free 8-iso-PGF(2 alpha) the levels in plasma increased significantly up to 2 h and remained at this level until death among the non-survivors. This was apparently different from the survivors where the 8-iso-PGF(2 alpha) levels increased to its height at 1 h, then decreased to the basal levels after 5 h, Thus, free radical and cyclooxygenase catalyzed oxidation of arachidonic acid occurs during endotoxemia, Free radical dependent oxidative injury following endotoxin induced inflammation may be the major cause of organ failure and increased mortality. (C) 1998 Federation of European Biochemical Societies.