AN IMPROVED PROCEDURE FOR THE METHYLATION ANALYSIS OF OLIGOSACCHARIDES AND POLYSACCHARIDES
AN IMPROVED PROCEDURE FOR THE METHYLATION ANALYSIS OF OLIGOSACCHARIDES AND POLYSACCHARIDES
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DOI：
10.1016/0008-6215(84)85106-x
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发表时间：
1984-01-01
影响因子：
3.1
通讯作者：
STONE, BA
中科院分区：
文献类型：
--
作者：
HARRIS, PJ;HENRY, RJ;STONE, BA
介绍了一种改进的寡糖和多糖甲基化分析方法。对该程序中的步骤进行了检查，并针对定量回收率和速度进行了优化。通过使用[14C]甲基碘显示甲基化完全。所有操作都在同一管中进行，并且不需要浓缩含有甲基化糖的乙酰化糖醇的溶液，从而使由于挥发造成的损失最小化。该方法是方便的，给出了甲基化糖的乙酰化糖醇的高回收率，并且允许在一个工作日内完成寡糖或多糖的糖基键的分析。广泛的低聚糖和多糖甲基化通过这个程序。
An improved procedure for the methylation analysis of oligosaccharides and polysaccharides is described. Steps in the procedure were examined and optimized for quantitative recovery and speed. Methylation was shown to be complete by using [14C]methyl iodide. All operations were performed in the same tube and the need to concentrate solutions containing acetylated alditols of methylated sugars was eliminated, thus minimizing losses due to volatilization. The method is convenient, gives high recoveries of acetylated alditols of methylated sugars, and allows analysis of the glycosyl linkages of oligo- or polysaccharides to be completed within a working day. A wide range of oligo- and polysaccharides were methylated by this procedure.