Integration of a splicing regulatory network within the meiotic gene expression program of Saccharomyces cerevisiae
Integration of a splicing regulatory network within the meiotic gene expression program of Saccharomyces cerevisiae
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DOI：
10.1101/gad.1977410
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发表时间：
2010-12-01
影响因子：
10.5
通讯作者：
Ares, Manuel, Jr.
中科院分区：
文献类型：
--
作者：
Munding, Elizabeth M.;Igel, A. Haller;Ares, Manuel, Jr.
剪接调控网络是真核生物基因表达程序的重要组成部分，但很少有人知道它们是如何与转录调控网络整合成连贯的基因表达程序。在这里，我们定义了MER1剪接调控网络，并研究其在芽殖酵母减数分裂过程中的基因表达程序中的作用。Mer1p剪接因子仅促进四种前mRNA的剪接。所有四个Mer1p响应基因也需要Nam8p的剪接激活Mer1p，然而，其他基因需要Nam8p，而不是Mer1p，暴露出一个重叠的减数分裂剪接网络控制Nam8p。MER1 mRNA和四种Mer1p底物前mRNA中的三种由转录调节因子Ume6p诱导。相对于Ume6p控制下的其他基因，这种不寻常的排列延迟了Mer1p响应基因的表达。Mer1p响应基因的产物是启动和完成重组以及激活Ndt80p所必需的，Ndt80p是程序中后续步骤所需的转录网络的激活剂。因此，MER1剪接调控网络介导减数分裂基因表达程序中UME6和NDT 80转录调控网络之间的依赖关系。这项研究揭示了剪接调控网络可以交织在真核基因表达程序的转录调控网络。
Splicing regulatory networks are essential components of eukaryotic gene expression programs, yet little is known about how they are integrated with transcriptional regulatory networks into coherent gene expression programs. Here we define the MER1 splicing regulatory network and examine its role in the gene expression program during meiosis in budding yeast. Mer1p splicing factor promotes splicing of just four pre-mRNAs. All four Mer1p-responsive genes also require Nam8p for splicing activation by Mer1p; however, other genes require Nam8p but not Mer1p, exposing an overlapping meiotic splicing network controlled by Nam8p. MER1 mRNA and three of the four Mer1p substrate pre-mRNAs are induced by the transcriptional regulator Ume6p. This unusual arrangement delays expression of Mer1p-responsive genes relative to other genes under Ume6p control. Products of Mer1p-responsive genes are required for initiating and completing recombination and for activation of Ndt80p, the activator of the transcriptional network required for subsequent steps in the program. Thus, the MER1 splicing regulatory network mediates the dependent relationship between the UME6 and NDT80 transcriptional regulatory networks in the meiotic gene expression program. This study reveals how splicing regulatory networks can be interlaced with transcriptional regulatory networks in eukaryotic gene expression programs.