FACTORS AFFECTING THE KINETICS OF LIGHT-EMISSION FROM CRUDE AND PURIFIED FIREFLY LUCIFERASE
FACTORS AFFECTING THE KINETICS OF LIGHT-EMISSION FROM CRUDE AND PURIFIED FIREFLY LUCIFERASE
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DOI：
10.1016/0003-2697(79)90204-5
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发表时间：
1979-01-01
影响因子：
2.9
通讯作者：
MCELROY, WD
中科院分区：
文献类型：
--
作者：
DELUCA, M;WANNLUND, J;MCELROY, WD
用粗制和纯化的萤火虫荧光素酶测定0.2 pmol-2 μ mol的ATP。在这个ATP浓度范围内，光发射的动力学有很大的变化。在ATP浓度最低时，光发射上升到最大值，并在一分钟或更长时间内保持恒定。随着ATP浓度的增加，峰值光强增加，光衰减速率显著增加。这对于粗酶和纯化的酶都是如此。高浓度的砷酸钠和其他盐抑制峰值光发射，并防止由于产物抑制引起的光强度衰减。几乎任何类型的动力学都可以通过操纵实验条件来获得。
Crude and purified firefly luciferase was used to assay ATP from 0.2 pmol-2 .mu.mol. Over this range of ATP concentrations there is a large change in the kinetics of light emission. At the lowest concentrations of ATP, light emission rises to a maximum and remains constant for a minute or longer. As the concentration of ATP is increased, the peak light intensity increases and the decay rate of light increases significantly. This is true for both the crude and the purified enzyme. High concentration of sodium arsenate and other salts inhibit the peak light emission and prevent the decay in light intensity which is due to product inhibition. Almost any type of kinetics can be obtained by manipulating the experimental conditions.