O-289 What makes people try to conceive? Findings from the international fertility decision-making study [Abstract]
O-289 What makes people try to conceive? Findings from the international fertility decision-making study [Abstract]
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O-289 是什么促使人们尝试怀孕？
DOI：
10.1093/humrep/de.25.s1.69
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发表时间：
2010
影响因子：
6.1
通讯作者：
C. Harrison
中科院分区：
文献类型：
--
作者：
J. Boivin;L. Bunting;I. Tsibulsky;N. Kalebic;C. Harrison
一种遗传基础。RT-PCR检测证实了激活素的存在。此外，还用定量RT-PCR和Western blotting对P450芳香酶(ARO)进行了研究。结果：永生化黄素化颗粒细胞(HLGCs)和永生化非黄素化颗粒细胞(HGRCs)表达ARO、FSH-R和LHRmRNA。Forskolin和8-BrcAMP能迅速刺激HLGCs和HGCs分泌E2和P4，而FSH和hCG对类固醇激素的诱导作用较弱。加入Forsklin和8-BR-cAMP后，HGCs的ARO增加，而成纤维细胞则不增加。HLGCs和HGCs表达BMP-2、-4、-5、-6、-7和-15，而HLGCs表达BMP-6、-7和-15较少。HLGCs和HGCs表达BMP受体I型、Ib型和II型。结论：建立了具有类固醇生成活性的人黄体颗粒细胞和非黄体颗粒细胞永生化细胞系。我们推测，这些HGCs似乎被定性为FSH依赖生长之前的不成熟阶段。这些细胞系将有助于我们研究人类卵巢的卵泡发生。简介：ART婴儿的出生性别比(SSR，男婴比例)因ART程序的不同而不同。ART程序是否对SSR有任何重大影响尚有争议。与其他国家出生的婴儿相比，ART婴儿的平均胎龄更短，平均出生体重更低。在意大利、西班牙、墨西哥和巴西，与其他国家相比，个人和关系准备/稳定的影响力较小(p<；.001)。在经济安全感的高度影响上，各国并无不同。结论：研究结果表明，一些决定因素与成家有关，如强烈的想要孩子或需要经济上有保障。然而，其他人的影响因国家而异，在男女之间也不同：需要孩子才能获得终身幸福，经历着生育的社会压力，或者想要遵守这些社会规范，并不是所有国家都一样。决定因素的异质性意味着，改善生育率下降的政策需要处理共同的障碍(例如，欧洲关于经济先决条件的政策)和决定因素，特别是在一些国家(例如，加强意大利社会对儿童的需求)。出乎意料的是，男性比女性(在所有国家)更了解和更愿意服从家庭和姻亲的生育压力，尽管他们对孩子的渴望和需求较弱。对此的一种有争议的解释是，男性采取生育策略旨在满足社会义务，而不是满足个人需求。应该开始调查和治疗。这项研究提供了一个基础，以确定从夫妇一直试图怀孕的时间周期中可以推断出什么关于夫妇的生育能力。演讲将对概率在构思过程中的作用提供重要的见解。产生的结果似乎是稳健的。这一办法有可能有助于利用非受孕期进行生育率评估的决策支持工具。与对照组相比。在这组患者中，我们没有发现出生体重或胎龄的差异。在目前正在进行的一项全国性研究中，所有这些结果都将在更大的研究人群中重新评估，并与治疗历史相关。参与这项全国性研究的中心有：阿姆斯特丹学术医学中心、鹿特丹伊拉斯谟医学中心、莱斯大学医学中心、格罗宁根大学医学中心、圣拉德布·奈梅亨大学医学中心、乌得勒支大学医学中心和阿姆斯特丹弗吉尼亚大学医学中心。这项研究得到了荷兰癌症协会的资助(批准号：VU 2006-3622)和《Stichting Kika》(无儿童癌症基金会)。胎龄、较高的平均出生体重和与一般人口出生相似的SSR。虽然ART对SSR的影响机制尚不清楚，但数据显示，与普通人群出生相比，IVF+BL导致更高的男生率，而ICSI+CL生更多的女婴。与卵裂期胚胎移植后出生的独生子女相比，胚泡移植后出生的独生子女具有更高的SSR、更长的GA和更高的BWT。
a genetic basis. and activin was confirmed by RT-PCR. Furthermore, p450 aromatase (ARO) was studied with quantitative RT-PCR and Western blotting. results: Immortalized luteinized granulosa cells (HLGrCs) and immortalized non-luteinized granulosa cells (HGrCs) expressed ARO, FSH-R and LH-R mRNA. Forskolin and 8-Br-cAMP stimulated secretion of E 2 and P 4 rapidly in HLGrCs and HGrCs, while stimulation with FSH and hCG were less inducible of steroidogenesis. ARO was increased in HGrCs, not fibroblasts as negative control, with forskolin and 8-Br-cAMP. BMP-2, -4, -5, -6, -7 and -15 were expressed by HLGrCs and HGrCs, while the expression of BMP-6, -7 and -15 was less in HLGrCs. BMP receptor typeIA, typeIB and typeII were expressed in HLGrCs and HGrCs. conclusions: We established the immortalized cell lines derived from human luteinized granulosa cells and non-luteinized granulosa cells, which possess steroidogenesis activity. We speculate that these HGrCs seem to be character-ized as immature stages before FSH-dependent growth. These cell lines will help us to investigate the folliculogenesis of the human ovary. introduction: The sex ratio at birth (SSR, proportion of male births) of ART babies varies depending on the ART procedures. Whether the ART procedures have any significant effect on SSR is debatable. ART babies also have shorter mean gestational ages and lower mean birthweights, compared to babies born than in other countries. In Italy, Spain, Mexico and Brazil personal and relation- ship readiness/stability was less influential (p < .001) than in other countries. Countries did not differ on the high influence of feeling economically secure. conclusion: The results demonstrate that some decisional factors have a uni- versal association with starting families, for example the strong desire for children or the need to be financially secure. However, the influence of others varies from country to country and between men and women: needing children for life-long happiness, experiencing social pressure to have children or want-ing to conform to these social norms are not equally present in all countries. Heterogeneity in decisional factors means that policies to improve declining fertility rates need to tackle both shared barriers (e.g., European policies on economic preconditions) and decisional factors particularly present in some countries (e.g., reinforcing societal need for children in Italy). Unexpectedly, men were more aware of and more willingly to comply with family and in-law pressure to have children than women (across all countries) despite a weaker desire and need for children. A controversial interpretation of this would be that men adopt a childbearing strategy aimed at meeting social obligation rath-er than satisfying personal need. should begin investigation and treatment. This study provides a basis for determining what can be deduced about a couple’s fertility from the pe-riod of time which they have been trying to conceive. The presentation will provide important insights into the role of probability in the conception process. yields results that appear to be robust. This approach has the potential to contribute to decision support tools which make use of the duration of non-conception for fertility assessment. compared to controls. In this group of patients, we did not find differences in birth weight or gestational age. In a nationwide study a , which is currently being performed, all these outcomes will be re-evaluated in a larger study population and in relation to treatment history. a Participating centers in the nationwide study: Academic Medical Center Amsterdam, Erasmus Medical Center Rotterdam, Leids University Medical Center, University Medical Center Groningen, University Medical Center St Radboud Nijmegen, University Medical Center Utrecht and VU University Medical Center Amsterdam. This study was financially supported by the Dutch Cancer Society (grant no. VU 2006-3622) and by ‘Stichting KiKa’ (Foundation Children Cancerfree). gestational ages, higher mean birthweights and similar SSR to general population births. Although the mechanism of the impact on the SSR by ART is unclear, the data shows that IVF + BL leads to a higher rate of male births, compared to general population births, while ICSI + CL has more female births. Singletons born following blastocyst transfer have a higher SSR, a longer GA and a higher BWT, compared to the singletons born following cleavage stage embryo transfer.