Mass spectrometry in plant proteomic analysis
Mass spectrometry in plant proteomic analysis
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DOI：
10.1080/11263501003764392
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发表时间：
2010-01-01
期刊：
影响因子：
2
通讯作者：
Shaw，P. J.
中科院分区：
文献类型：
--
作者：
Colas，I.;Koroleva，O.;Shaw，P. J.
目前蛋白质组学的革命是由非常敏感的质谱联用微毛细管液相色谱、蛋白质混合物的特异性蛋白质水解和能够从测序基因组中搜索大量质量测量结果的软件相结合而产生的。基因组后植物生物学的挑战包括蛋白质功能的表征、蛋白质复合物的翻译后修饰和组成，以及细胞内区室（细胞器的蛋白质组）中蛋白质补体的破译。本文综述了目前用于植物蛋白质组学的质谱分析方法，并讨论了用于植物蛋白质复合物纯化和蛋白质组学分析的各种标记策略。缩写：BCCD，生物素羧基载体蛋白域；钙调素结合蛋白；CID：碰撞诱导解离；ESI，电喷雾电离；EST，表达序列标签；傅里叶变换离子回旋共振；绿色荧光蛋白；GST，谷胱甘肽S转移酶；哈,血凝素;HILEP，全株水培同位素标记；他,组氨酸;HPB HA-PreScission-Biotin;高效液相色谱法；同位素编码亲和标签；同位素编码蛋白标签；iTRAQ，等压标签，用于相对和绝对定量；LC，液相色谱法；MALDI，基质辅助激光解吸电离；MBP，麦芽糖结合蛋白；质谱法；SDS - PAGE，十二烷基硫酸钠-聚丙烯酰胺凝胶电泳；细胞培养中氨基酸稳定同位素标记；植物稳定同位素标记；喉炎、链霉亲和素;TAP，串联亲和纯化；TBP, TATA - box - binding protein；TOF、飞行时间优先车道;超高效液相色谱法
The current revolution in proteomics has been generated by the combination of very sensitive mass spectrometers coupled to microcapillary liquid chromatography, specific proteolysis of protein mixtures and software that is capable of searching vast numbers of mass measurements against predicted peptides from sequenced genomes. The challenges of post‐genomic plant biology include characterization of protein function, post‐translational modifications and composition of protein complexes as well as deciphering protein complements in intracellular compartments – proteomes of cell organelles. In this review we summarize the current mass spectrometry methods currently being used in plant proteomics and discuss the various tagging strategies that are being used for purification and proteomic analysis of plant protein complexes.Abbreviations:BCCD, biotin carboxyl carrier protein domain; CBP, calmodulin‐binding protein; CID, collision‐induced dissociation; ESI, electrospray ionization; EST, expressed sequence tag; FT‐ICR, Fourier transform ion cyclotron resonance; GFP, green fluorescent protein; GST, glutathione S‐transferase; HA, haemagglutinin; HILEP, hydroponic isotope labelling of entire plants; His, histidine; HPB, HA–PreScission–Biotin; HPLC, high‐performance liquid chromatography; ICAT, isotope‐coded affinity tags; ICPL, isotope‐coded protein label; iTRAQ, isobaric tag for relative and absolute quantification; LC, liquid chromatography; MALDI, matrix‐assisted laser desorption ionization; MBP, maltose‐binding protein; MS, mass spectrometry; SDS‐PAGE, sodium dodecyl sulphate‐polyacrylamide gel electrophoresis; SILAC, stable isotope labelling with amino acids in cell culture; SILIP, stable isotope labellingin planta; Strep, streptavidin; TAP, tandem affinity purification; TBP, TATA‐box‐binding protein; TOF, time‐of‐flight; UPLC, ultraperformance liquid chromatography