Separation of plasma high-density lipoprotein subclasses by a combined precipitation method using polyethylene glycol 6000 and dextran sulphate.
Separation of plasma high-density lipoprotein subclasses by a combined precipitation method using polyethylene glycol 6000 and dextran sulphate.
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使用聚乙二醇 6000 和硫酸葡聚糖通过组合沉淀法分离血浆高密度脂蛋白亚类。
DOI：
10.3109/00365518409083812
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发表时间：
1984
影响因子：
2.1
通讯作者：
J. Viikari
中科院分区：
文献类型：
--
作者：
B. Lundberg;S. Högström;P. Pietiläinen;M. Suominen;J. Viikari
本文介绍了一种快速、廉价的分离HDL亚类的沉淀方法。其开始于用PEG-6000沉淀含有载脂蛋白B的脂蛋白，之后用硫酸葡聚糖-Mg 2+沉淀HDL 2。发现0.72 g/l硫酸葡聚糖的浓度适合于HDL亚类的分离。通过沉淀获得的HDL亚类在密度梯度离心时得到适当的密度分布。本法与连续离心法和密度梯度离心法所得结果相关性良好，r分别为0.99和0.72。
A fast and cheap precipitation method for the separation of HDL subclasses been evaluated. It starts with precipitation of apo-B containing lipoproteins with PEG-6000 after which HDL2 is precipitated with dextran sulphate-Mg2+. A concentration of 0.72 g/l dextran sulphate was found to be suitable for the separation of HDL subclasses. HDL subclasses obtained by precipitation gave proper density profiles at density gradient centrifugations. The correlations between results obtained by this method and by sequential and density gradient centrifugation are good, r = 0.99 and 0.72, respectively.