Antioxidative nanofullerol prevents intervertebral disk degeneration.
Antioxidative nanofullerol prevents intervertebral disk degeneration.
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DOI：
10.2147/ijn.s60853
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发表时间：
2014
影响因子：
8
通讯作者：
Li X
中科院分区：
文献类型：
--
作者：
Yang X;Jin L;Yao L;Shen FH;Shimer AL;Li X
令人信服的证据表明，活性氧（ROS）在椎间盘退变中起着关键作用。在水溶液中制备的富勒醇纳米颗粒已被证明具有出色的清除ROS的能力。本研究采用体外和体内模型研究富勒醇预防椎间盘退变的疗效。在体外实验中，使用促氧化剂H2 O2或炎性细胞因子白细胞介素（IL）-1β诱导包封在藻酸盐珠中的人髓核细胞的退化表型，并在培养基中加入富勒醇。在动物实验中，建立兔的纤维环穿刺模型，并将富勒醇注入椎间盘。结果表明，富勒醇能显著降低H_2O_2诱导的细胞毒性和细胞内ROS水平。富勒醇也能抑制IL-1β诱导的培养液中一氧化氮的生成。基因表达谱和生化分析表明富勒醇能有效逆转H2 O2或IL-1β引起的基质降解。动物研究表明，椎间盘内注射富勒醇可防止椎间盘退变，增加水和蛋白多糖含量，抑制异位骨形成。这些结果表明，抗氧化富勒醇可能有一个潜在的椎间盘退变的治疗应用。
Compelling evidence suggests that reactive oxygen species (ROS) play a pivotal role in disk degeneration. Fullerol nanoparticles prepared in aqueous solution have been demonstrated to have outstanding ability to scavenge ROS. In this report, in vitro and in vivo models were used to study the efficacy of fullerol in preventing disk degeneration. For in vitro experiments, a pro-oxidant H2O2 or an inflammatory cytokine interleukin (IL)-1β was employed to induce degenerated phenotypes in human nucleus pulposus cells encapsulated in alginate beads, and fullerol was added in the culture medium. For the animal study, an annulus-puncture model with rabbit was created, and fullerol was injected into disks. It was shown that cytotoxicity and cellular ROS level induced by H2O2 were significantly diminished by fullerol. IL-1β-induced nitric oxide generation in culture medium was suppressed by fullerol as well. Gene-profile and biochemical assays showed that fullerol effectively reversed the matrix degradation caused by either H2O2 or IL-1β. The animal study delineated that intradiskal injection of fullerol prevented disk degeneration, increasing water and proteoglycan content and inhibiting ectopic bone formation. These results suggest that antioxidative fullerol may have a potential therapeutic application for disk degeneration.