Direct detection of blood in fingermarks by MALDI MS profiling and Imaging
Direct detection of blood in fingermarks by MALDI MS profiling and Imaging
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DOI：
10.1016/j.scijus.2013.12.004
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发表时间：
2014-03-01
影响因子：
1.9
通讯作者：
Francese, S.
中科院分区：
文献类型：
--
作者：
Bradshaw, R.;Bleay, S.;Francese, S.
确定指纹中的血迹是刑事调查中的重要情报，因为它有助于重建犯罪现场发生的事件。已经报道了各种方法，并且目前用于检测血液的存在，包括光学、光谱和化学显影方法。然而，大多数方法只给出了一个迹象，血液可能存在，因此，这些方法被描述为推定tests.Here，我们显示使用基质辅助激光解吸电离质谱分析和成像（MALDI MSP和MALDI MSI），通过特异性检测血红素和血红蛋白的分子，通过它们的质荷比，用于确定指纹中血液的存在。此外，初步的实验表明，该技术是兼容的，目前采用的其他方法，以提高血液中的指纹（或血液污染）。所开发的协议的犯罪现场的血液痕迹的应用，证明了在日常工作中使用这种技术的可行性。这些发现开辟了一条新的研究路线，用于开发强大的MALDI MSP和MALDI MSI协议，用于血渍的检测和化学成像。(C)2013年，《法医科学》杂志社。由Elsevier爱尔兰有限公司出版。保留所有权利。
The determination of the presence of blood in fingermarks constitutes important intelligence in a criminal investigation as it helps to reconstruct the events that have taken place at a scene of crime. Various methodologies have been reported and are currently employed for the detection of the presence of blood including optical, spectroscopic and chemical development approaches. However, most methods only give an indication that blood may be present and, therefore, these methods are described as presumptive tests.Here we show the use of Matrix-Assisted Laser Desorption Ionisation Mass Spectrometry Profiling and Imaging (MALDI MSP and MALDI MSI) for the determination of the presence of blood in fingermarks by specifically detecting the molecules of haem and haemoglobin through their mass-to-charge ratios. Furthermore, preliminary experiments are shown which demonstrate that this technology is compatible with other methods currently employed for enhancing fingermarks in blood (or contaminated by blood). The application of the developed protocols to a crime scene blood trace, demonstrates the feasibility of using this technology in routine casework. These findings open up a new line of research for the development of robust MALDI MSP and MALDI MSI protocols for the detection and chemical imaging of bloodied marks. (C) 2013 Forensic Science Society. Published by Elsevier Ireland Ltd. All rights reserved.