Deep cis-regulatory homology of the butterfly wing pattern ground plan
Deep cis-regulatory homology of the butterfly wing pattern ground plan
复制标题
DOI：
10.1126/science.abi9407
复制
发表时间：
2022-10-20
期刊：
影响因子：
56.9
通讯作者：
Reed, Robert D.
中科院分区：
文献类型：
--
作者：
Mazo-Vargas, Anyi;Langmueller, Anna M.;Reed, Robert D.
蝴蝶翅膀的图案来自于一个高度保守的发展基础计划，但却多种多样，进化迅速。基因调控结构如何适应深度同源性和适应性变化还知之甚少。为了解决这一问题，我们的特点是顺式调控的颜色模式基因WntA在nymphalid蝴蝶进化。使用测序（ATAC-seq）和跨46个顺式调控元件在5个物种的体内缺失转座酶可访问的染色质的比较分析揭示了深的同源性的地面计划决定序列，除了在帝王蝶。此外，非编码缺失表现出积极和消极的调节作用，往往是广泛的性质。我们的研究结果为预测快速增强子周转的模型提供了很少的支持，并表明，祖先的多功能非编码元件可以成为快速进化的性状系统的基础。
Butterfly wing patterns derive from a deeply conserved developmental ground plan yet are diverse and evolve rapidly. It is poorly understood how gene regulatory architectures can accommodate both deep homology and adaptive change. To address this, we characterized the cis-regulatory evolution of the color pattern gene WntA in nymphalid butterflies. Comparative assay for transposase-accessible chromatin using sequencing (ATAC-seq) and in vivo deletions spanning 46 cis-regulatory elements across five species revealed deep homology of ground plan-determining sequences, except in monarch butterflies. Furthermore, noncoding deletions displayed both positive and negative regulatory effects that were often broad in nature. Our results provide little support for models predicting rapid enhancer turnover and suggest that deeply ancestral, multifunctional noncoding elements can underlie rapidly evolving trait systems.