Simultaneous determination of the main metabolites in rice leaves using capillary electrophoresis mass spectrometry and capillary electrophoresis diode array detection
Simultaneous determination of the main metabolites in rice leaves using capillary electrophoresis mass spectrometry and capillary electrophoresis diode array detection
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DOI：
10.1111/j.1365-313x.2004.02187.x
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发表时间：
2004-10-01
期刊：
影响因子：
7.2
通讯作者：
Tomita, M
中科院分区：
文献类型：
--
作者：
Sato, S;Soga, T;Tomita, M
使用常规化学分析进行初级代谢物的代谢组学研究需要高通量方法。化学衍生是气相色谱分离的先决条件，液相色谱分离和核磁共振检测系统需要大量的样品。最近，我们开发了一种毛细管电泳-质谱（CE-MS）技术，该技术可以同时定量大量的初级代谢产物，仅使用少量的样品，并且不需要任何化学衍生。并行使用毛细管电泳-二极管阵列检测器（CE-DAD）系统进一步使几乎所有的水溶性细胞内代谢物进行分析。我们证明，与水稻叶片，一个简单而快速的方法CE分析样品制备，使用这种方法，我们已经成功地测量了88个主要代谢产物参与糖酵解，三羧酸循环，戊糖磷酸途径，光呼吸和氨基酸生物合成的水平。
The study of the metabolomics of primary metabolites using conventional chemical analyses requires a high-throughput method. Chemical derivatizations are a prerequisite for gas-chromatographic separation, and a large sample quantity is needed for liquid-chromatographic separation and nuclear magnetic resonance detection systems. Recently, we have developed a capillary electrophoresis-mass spectrometry (CE-MS) technology that can simultaneously quantify a large number of primary metabolites, using only a small quantity of samples, and without any chemical derivatizations. Parallel use of a capillary electrophoresis-diode array detector (CE-DAD) system further enables almost all water-soluble intracellular metabolites to be analyzed. We demonstrate, with rice leaves, a simple and rapid method of sample preparation for CE analysis; using this method, we have successfully measured the levels of 88 main metabolites involved in glycolysis, the tricarboxylic acid cycle, the pentose phosphate pathway, photorespiration, and amino acid biosynthesis.