A multi-scale map of cell structure fusing protein images and interactions.
A multi-scale map of cell structure fusing protein images and interactions.
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DOI：
10.1038/s41586-021-04115-9
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发表时间：
2021-12
期刊：
影响因子：
64.8
通讯作者：
中科院分区：
文献类型：
--
作者：
The cell is a multi-scale structure with modular organization across at least four orders of magnitude. Two central approaches for mapping this structure—protein fluorescent imaging and protein biophysical association—each generate extensive datasets, but of distinct qualities and resolutions that are typically treated separately. Here we integrate immunofluorescence images in the Human Protein Atlas with affinity purifications in BioPlex to create a unified hierarchical map of human cell architecture. Integration is achieved by configuring each approach as a general measure of protein distance, then calibrating the two measures using machine learning. The map, known as the multi-scale integrated cell (MuSIC 1.0), resolves 69 subcellular systems, of which approximately half are to our knowledge undocumented. Accordingly, we perform 134 additional affinity purifications and validate subunit associations for the majority of systems. The map reveals a pre-ribosomal RNA processing assembly and accessory factors, which we show govern rRNA maturation, and functional roles for SRRM1 and FAM120C in chromatin and RPS3A in splicing. By integration across scales, MuSIC increases the resolution of imaging while giving protein interactions a spatial dimension, paving the way to incorporate diverse types of data in proteome-wide cell maps.
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影响因子：
64.8
作者：
Pomeranz Krummel, Daniel A;Oubridge, Chris;Leung, Adelaine K W;Li, Jade;Nagai, Kiyoshi
通讯作者：
Nagai, Kiyoshi
DOI：
10.1145/2939672.2939754
发表时间：
2016-08
期刊：
KDD : proceedings. International Conference on Knowledge Discovery & Data Mining
影响因子：
--
作者：
Grover A;Leskovec J
通讯作者：
Leskovec J
影响因子：
5.3
作者：
Deckert, Jochen;Hartmuth, Maus;Luehrmann, Reinhard
通讯作者：
Luehrmann, Reinhard
DOI：
10.1007/978-1-4939-7204-3_14
发表时间：
2017-01-01
期刊：
MRNA PROCESSING: METHODS AND PROTOCOLS
影响因子：
--
作者：
Van Nostrand, Eric L.;Nguyen, Thai B.;Yeo, Gene W.
通讯作者：
Yeo, Gene W.
影响因子：
4.5
作者：
Chaudhuri, Sujan;Vyas, Keyur;Mazumder, Barsanjit
通讯作者：
Mazumder, Barsanjit