Anion-exchange high performance liquid chromatography method for the quantitation of nucleotides in human blood cells.
Anion-exchange high performance liquid chromatography method for the quantitation of nucleotides in human blood cells.
复制标题
阴离子交换高效液相色谱法用于定量人血细胞中的核苷酸。
DOI：
10.1016/0009-8981(85)90145-7
复制
发表时间：
1985
期刊：
影响因子：
--
通讯作者：
A. V. van Gennip
中科院分区：
文献类型：
--
作者：
D. de Korte;W. Haverkort;D. Roos;A. V. van Gennip
An anion-exchange high performance liquid chromatography (HPLC) method is described for the quantitation of intracellular purine and pyrimidine nucleotides. With an ammonium phosphate salt and pH gradient, complete separation is achieved of all major nucleotides and several interfering substances, such as dehydroascorbic acid and NAD. For optimal resolution of the monophosphates, strict control of the equilibration pH is essential. To prevent interference by a degradation product of NADPH with the determination of GDP, the pH of the high-ionic strength buffer has to be in the range of 4.9-5.0. The use of radially compressed, prepacked cartridges filled with Partisil-10 SAX appeared to be a fast and cheap alternative for expensive stainless-steel columns. The use of ammonium phosphate buffers, in combination with precolumns filled with pellicular silica and SAX resin, and interim EDTA washes prevents baseline shift. This allows analysis at 0.01 Absorbance Units Full Scale during the entire column lifetime (about 180 analyses), which is sufficiently sensitive for the quantitation of low levels of nucleotides, especially when the amount of sample is limited.The usefulness of the presented Chromatographie system is demonstrated by the quantitation of the nucleotides in extracts of lymphocytes and neutrophils from the blood of healthy human donors. With this method nucleotide concentrations were measured, with a within-assay variation of 5–10% and an inter-donor variation of 10%.
通过等度反相高效液相色谱从粒细胞中快速分离核苷酸。
DOI：
10.1016/s0378-4347(00)86053-3
发表时间：
1982
期刊：
Journal of chromatography
影响因子：
--
作者：
Rao,GH;Peller,JD;Richards,KL;McCullough,J;White,JG
通讯作者：
White,JG
正常人和慢性淋巴细胞白血病患者单核细胞的核糖核苷酸含量：慢性淋巴细胞白血病淋巴细胞中烟酰胺腺嘌呤二核苷酸浓度增加。
影响因子：
11.2
作者：
Liebes,LF;Krigel,RL;Conklyn,M;Nevrla,DR;Silber,R
通讯作者：
Silber,R