Ameloblastic carcinoma ex ameloblastoma: report of a case-possible involvement of CpG island hypermethylation of the p16 gene in malignant transformation
Ameloblastic carcinoma ex ameloblastoma: report of a case-possible involvement of CpG island hypermethylation of the p16 gene in malignant transformation
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DOI：
10.1016/j.tripleo.2006.01.021
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发表时间：
2007-01-01
期刊：
影响因子：
--
通讯作者：
Kaku, Tohru
中科院分区：
文献类型：
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作者：
Abiko, Yoshihiro;Nagayasu, Hiroki;Kaku, Tohru
造釉细胞癌在更新的世界卫生组织（WHO）分类中被描述为一种罕见的恶性病变。符合WHO标准的成釉细胞癌可能是由于既存良性成釉细胞瘤的恶性变化（成釉细胞瘤前癌）或原发性恶性成釉细胞瘤，而之前没有普通的成釉细胞瘤（新发癌）。我们报告一例成釉细胞癌前成釉细胞瘤，并探讨如何这种情况下进行恶性转化。分别从肿瘤的石蜡切片中的良性和恶性区域提取DNA。直接测序显示p53基因外显子5 - 8无突变。p16基因CpG岛的高甲基化在肿瘤的恶性部分被检测到。结果表明，p16基因的甲基化可能参与了本例成釉细胞瘤的恶性转化。
Ameloblastic carcinoma is described in the updated World Health Organization (WHO) classification as a rare malignant lesion. Ameloblastic carcinoma meeting the WHO criteria may arise either as a result of malignant change in a pre-existing benign ameloblastoma (carcinoma ex ameloblastoma) or as a primary malignant ameloblastoma not preceded by an ordinary ameloblastorna (de novo carcinoma). We report a case of ameloblastic carcinoma ex ameloblastoma and examine how this case underwent malignant transformation. The DNA was extracted separately from benign and malignant areas in paraffin sections of the tumor. Direct sequencing showed no genetic mutation of exons 5-8 of the p53 gene. Hypermethylation of CpG islands of the p16 gene was detected in the malignant parts of the tumor. The results indicate that hypermethylation of p16 may have been involved in the malignant transformation of the ameloblastoma in the present case.