NUMBERS, DIVERSITY, AND MORPHOLOGICAL-CHARACTERISTICS OF AEROBIC, CHEMOHETEROTROPHIC BACTERIA IN DEEP SUBSURFACE SEDIMENTS FROM A SITE IN SOUTH-CAROLINA
NUMBERS, DIVERSITY, AND MORPHOLOGICAL-CHARACTERISTICS OF AEROBIC, CHEMOHETEROTROPHIC BACTERIA IN DEEP SUBSURFACE SEDIMENTS FROM A SITE IN SOUTH-CAROLINA
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DOI：
10.1080/01490458909377848
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发表时间：
1989-01-01
影响因子：
2.3
通讯作者：
BALKWILL, DL
中科院分区：
文献类型：
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作者：
BALKWILL, DL
通过直接显微镜、活细胞计数、平板菌落形态分析和分离菌株的细胞形态分析，对南卡罗来纳州一个地点的深层含水层和其他地下沉积物(深度达265m)原生的好氧、化学异养细菌进行了表征。在所有输导性含水层沉积物中都存在大量活细菌(105-108个/克)，而且它们的数量不会随着深度的增加而减少。在非传播性限制层中检测到的细菌较少(<；103/g)。在稀释液中获得的活菌数最多，但大多数含水层沉积物中10-50%的细菌也在营养丰富的浓缩液中快速生长(表明具有高度的代谢灵活性)。大多数细菌是中温细菌；检测到的嗜冷菌或嗜热菌相对较少(<；103/g；在许多情况下，没有)。细菌区系多样(大多数含水层沉积物的计数板上有11-62种不同的菌落类型)。多样性并没有随着深度的增加而减少，但微生物区系的组成(基于菌落分析)在不同的地质构造中差别很大。在计数平板上生长的可培养菌落中，几乎95%含有非链霉菌，其中80%以上是革兰氏阴性杆菌。通过浮选从含水层沉积物中释放出的薄膜的光学显微镜显示，存在分裂的细胞和微菌落，这意味着原位深层含水层微生物区系比以前在浅层含水层中看到的更活跃。
The aerobic, chemoheterotrophic bacteria indigenous to deep aquifers and other subsurface sediments (depths to 265 m) at a site in South Carolina were characterized by direct microscopy, enumeration of viable cells, analysis of colony morphologies on plates, and analysis of cell morphologies of isolated strains. Substantial numbers of viable bacteria (105-108/g) were present in all transmissive, aquifer sediments, and their numbers did not decrease with depth. Fewer bacteria (< 103/g) were detected in nontransmissive, confining layers. The highest viable counts were obtained on dilute media, but 10-50% of the bacteria in most aquifer sediments also grew rapidly on concentrated, nutrient-rich media (indicating a high degree of metabolic flexibility). Most of the bacteria were mesophilic; relatively few psychrophiles or thermophiles were detected (< 103/g; in many cases, none). The bacterial flora was diverse (11-62 distinct colony types on enumeration plates of most aquifer sediments). Diversity did not decrease with depth, but the composition of the microflora (based on colony analysis) varied extensively from one geological formation to another. Almost 95% of the platable colonies that grew on enumeration plates contained nonstreptomycete bacteria, more than 80% of which were gram-negative rods. Light microscopy of films released from aquifer sediments by flotation revealed the presence of dividing cells and microcolonies, thus implying that the in situ deep aquifer microflora was more metabolically active than that seen previously in shallow aquifers.