The Swi/Snf complex is important for histone eviction during transcriptional activation and RNA polymerase II elongation in vivo
The Swi/Snf complex is important for histone eviction during transcriptional activation and RNA polymerase II elongation in vivo
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DOI：
10.1128/mcb.00717-07
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发表时间：
2007-10-01
影响因子：
5.3
通讯作者：
Struhl, Kevin
中科院分区：
文献类型：
--
作者：
Schwabish, Marc A.;Struhl, Kevin
Swi/Snf 核小体重塑复合物由 DNA 结合激活蛋白招募，从而改变染色质结构以增加预起始复合物的形成和转录。在 SUC2 启动子处，Swi/Snf 复合物需要以独立于转录活性的方式驱逐组蛋白。 Swi/Snf 通过延伸 RNA 聚合酶 (Pol) II 穿过编码区，并且 swi2 突变体对影响 Pol 延伸的药物表现出敏感性。在 FACT 耗尽的细胞中，Swi/Snf 对于编码区内的内部起始很重要，这表明 Swi/Snf 对于 Pol II 延伸期间发生的组蛋白驱逐很重要。总而言之，这些观察结果表明 Swi/Snf 对于增强子处的组蛋白驱逐很重要，并且它还充当 Pol II 延伸因子。
The Swi/Snf nucleosome-remodeling complex is recruited by DNA-binding activator proteins, whereupon it alters chromatin structure to increase preinitiation complex formation and transcription. At the SUC2 promoter, the Swi/Snf complex is required for histone eviction in a manner that is independent of transcriptional activity. Swi/Snf travels through coding regions with elongating RNA polymerase (Pol) II, and swi2 mutants exhibit sensitivity to drugs affecting Pol elongation. In FACT-depleted cells, Swi/Snf is important for internal initiation within coding regions, suggesting that Swi/Snf is important for histone eviction that occurs during Pol II elongation. Taken together, these observations suggest that Swi/Snf is important for histone eviction at enhancers and that it also functions as a Pol II elongation factor.