Epstein-Barr virus induces an oxidative stress during the early stages of infection in B lymphocytes, epithelial, and lymphoblastoid cell lines
Epstein-Barr virus induces an oxidative stress during the early stages of infection in B lymphocytes, epithelial, and lymphoblastoid cell lines
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DOI：
10.1007/s11010-008-9755-z
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发表时间：
2008-06-01
影响因子：
4.3
通讯作者：
Attia, Hammadi
中科院分区：
文献类型：
--
作者：
Lassoued, Saloua;Ben Ameur, Randa;Attia, Hammadi
该研究调查了EB病毒感染对体外培养的人类细胞的氧化特征的直接影响。为此，选择了一组在其细胞和培养类型（上皮细胞或淋巴细胞;原代培养或连续细胞培养）中呈现异质性的人EBV靶细胞。这些细胞是纯化的人B淋巴细胞、DG 75、293和HepG 2细胞系。在感染的早期阶段（2，12，和24小时）通过测量丙二醛，脂质过氧化的最终产物，以及两种抗氧化酶：过氧化氢酶和超氧化物歧化酶的活性的氧化应激进行了评估。将所得结果与未处理细胞和与EBV无相互作用的K562细胞系的结果进行比较。不同靶细胞与EBV的孵育在纯化的B淋巴细胞DG 75和293中诱导氧化应激，但在HepG 2和K562中不诱导氧化应激。这种氧化应激通过MDA水平的增加（P < 0.05）而突出，其在加入病毒后2小时开始，并在12和24小时后持续。同时，过氧化氢酶和超氧化物歧化酶活性降低（P < 0.05），表明促进细胞抵抗活性氧（ROS）的分子机制发生了改变。通过病毒DNA PCR扩增评估的EBV感染效率在293和DG 75中得到证实，但在HepG 2中没有，这与它们的氧化特征完全一致。总之，EBV感染B和上皮细胞导致氧化应激的建立，这在病毒转化过程中起关键作用。
The study investigates the direct effect of Epstein-Barr virus infection on the oxidative profile of in vitro cultivated human cells. For this purpose, a panel of human EBV target cells presenting heterogeneity in their cellular and culture types (epithelial cells or lymphocytes; primary culture or continuous cell culture) was selected. These cells are purified human B lymphocytes, DG75, 293, and HepG2 cell lines. The oxidative stress was evaluated during the early stages of infection (2, 12, and 24 h) by measuring malondialdehyde, the end product of the lipid peroxidation, as well as the activities of two antioxidant enzymes: catalase and superoxide dismutase. The obtained results were compared with those of the untreated cells and the K562 cell line which has no interaction with EBV. The incubation of the different target cells with EBV induced an oxidative stress in the purified B lymphocytes, DG75, and 293, but not in HepG2 and K562. This oxidative stress was highlighted by an increase in MDA level (P < 0.05), which began 2 h after the addition of the virus and persisted after 12 and 24 h. Simultaneously, a decrease in catalase and superoxide dismutase activities was observed (P < 0.05), suggesting an alteration of the molecular mechanisms promoting cellular resistance to reactive oxygen species (ROS). The efficiency of EBV infection, assessed by viral DNA PCR amplification, was confirmed in 293 and DG75 but not in HepG2, which was in total concordance with their oxidative profiles. In conclusion, the EBV infection of B and epithelial cells leads to the establishment of an oxidative stress which can play a key role during the viral transformation.