Stability of stored methacholine solutions: study of hydrolysis kinetic by IP-LC
Stability of stored methacholine solutions: study of hydrolysis kinetic by IP-LC
复制标题
DOI：
10.1016/s0731-7085(01)00377-6
复制
发表时间：
2001-07-01
影响因子：
3.4
通讯作者：
Guyon, F
中科院分区：
文献类型：
--
作者：
Acar, V;Houri, JJ;Guyon, F
乙酰甲胆碱是一种强有力的胆碱能支气管收缩剂，用于支气管气道高反应性诊断。乙酰甲胆碱：在乙酸和S-甲基胆碱的水溶液中易水解。在目前的工作中，动力学或水解与不同的溶剂（水和磷酸盐缓冲盐水（PBS）pH 7.4）在不同的温度下已被研究，使用新开发的高效液相色谱。在4 degreesC，在乙酰甲胆碱氯化物溶液（50毫克/毫升）水解的动力学测定表明，无论是在水溶液或磷酸盐缓冲溶液超过40天的时间内没有水解。在30摄氏度。未缓冲的乙酰甲胆碱氯化物溶液的浓度保持不变，但缓冲的乙酰甲胆碱氯化物溶液在40天内降解高达5.5%。在40 ℃下，40天内，未缓冲的氯化乙酰甲胆碱浓度降解高达5%，而缓冲的氯化乙酰甲胆碱溶液降解高达10%。不同浓度的氯化乙酰甲胆碱溶液在医院药学中应合理使用。我们研究了乙酰甲胆碱的pH值和渗透压摩尔浓度：用医院药房可能使用的不同稀释剂制备的溶液，即去离子水、0.9% NaCl和PBS pH 7.4。我们已经证明，用去离子水以50 mg/ml制备并用PBS pH 7.4从5至40 mg/ml稀释的乙酰甲胆碱溶液是等渗的，并且可能可用于吸入试验以测量非特异性支气管高反应性。(C)2001爱思唯尔科技有限公司。保留所有权利。
Methacholine chloride is a powerful cholinergic bronchoconstrictor agent used during bronchial airway hyper-responsiveness diagnosis. Methacholine: is susceptible to hydrolysis in aqueous solutions in acetic acid and S-methyl choline. In the present work, kinetics or hydrolysis with different solvents (water and phosphate-buffered saline (PBS) pH 7.4) at different temperatures have been studied using a newly developed high-performance liquid chromatography. At 4 degreesC, kinetic determination of hydrolysis in methacholine chloride solutions (50 mg/ml) shows no hydrolysis in either aqueous or phosphate-buffered solutions over a 40-day period. At 30 degreesC. concentration of unbuffered methacholine chloride solutions remained unchanged, but buffered methacholine chloride solutions have degradation up to 5.5% over a 40-day period. At 40 degreesC, concentration of unbuffered methacholine chloride has degradation up to 5%, and buffered methacholine chloride solutions have degradation up to 10% over a 40-day period. Methacholine chloride solutions are susceptibly to be used in hospital pharmacy at different concentrations. We have studied pH and osmolality for methacholine: solutions prepared with different diluents potentially used in hospital pharmacies, i.e. deionized water, 0.9% NaCl and PBS pH 7.4. We have demonstrated that methacholine solutions prepared with deionized water at 50 mg/ml and diluted with PBS pH 7.4 from 5 to 40 mg/ml are isoosmotic and potentially available for inhalation tests to measure non-specific bronchial hyper-responsiveness. (C) 2001 Elsevier Science B.V. All rights reserved.