Recombinant human granulocyte colony-stimulating factor treatment improves macrophage suppression of granulocyte and macrophage growth after burn and burn wound infection
Recombinant human granulocyte colony-stimulating factor treatment improves macrophage suppression of granulocyte and macrophage growth after burn and burn wound infection
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DOI：
10.1097/00005373-199512000-00023
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发表时间：
1995-12-01
影响因子：
--
通讯作者：
Liu, H
中科院分区：
文献类型：
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作者：
Gamelli, RL;He, LK;Liu, H
烧伤或烧伤创面感染后粒细胞和巨噬细胞的产生明显减少，并进一步受到内毒素（ET）的影响，此外，巨噬细胞似乎是这种骨髓抑制的主要来源。我们试图确定重组人粒细胞集落刺激因子（rhG-CSF），一种能够改善实验性烧伤创面脓毒症后存活率的造血生长因子，改变的烧伤后巨噬细胞介导的骨髓抑制，接受15%总体表面积烧伤+/-铜绿假单胞菌感染（B或B + I）的雄性BDF 1小鼠组（n = 6至10）每天两次注射100 ng rhG-CSF。在第3天，将rhG-CSF处理或对照（5%葡萄糖水溶液）小鼠的腹膜诱导巨噬细胞（5 × 10（6）个细胞/mL）与+/- ET（300 ng/mL）孵育。将所得巨噬细胞上清液以1：10的体积加入到靶骨髓粒细胞-巨噬细胞祖细胞（GM-CFC）的培养物中。比较以不含巨噬细胞上清液的培养物的百分比表示的GM-CFC生长，并将GM-CFC数量的减少作为骨髓抑制的指标。与来自正常动物的巨噬细胞相比，从B和B + I动物获得的巨噬细胞减少了目标GM-CFC生长（B对正常动物p < 0.05）。此外，ET-刺激的巨噬细胞诱导所有三组的进一步骨髓抑制（p < 0.01）。对于所有组，与未处理的动物相比，来自粒细胞集落刺激因子处理的动物的巨噬细胞引起显著更少的骨髓抑制（p < 0.05至0.01）。粒细胞集落刺激因子管理烧伤后，除了作为一个直接的骨髓兴奋剂，也改变了巨噬细胞分泌的配置文件和改善巨噬细胞诱导的骨髓抑制。
Granulocyte and macrophage production after burn injury or burn wound infection is significantly reduced and further compromised by endotoxin (ET), Moreover, the macrophage seems to be the major source of this hone marrow suppression, We sought to determine if recombinant human granulocyte colony-stimulating factor (rhG-CSF), a hematopoietic growth factor that is capable of improving survival after experimental burn wound sepsis, altered postburn macrophage-mediated marrow suppression, Groups of male BDF1 mice (n = 6 to 10) receiving a 15% total body surface area burn +/- infection (B or B + I) with Pseudomonas aeruginosa were injected with 100 ng rhG-CSF twice daily. On day 3, peritoneal-elicited macrophages (5 x 10(6) cells/mL) from either rhG-CSF-treated or control(5% dextrose in water) mice were incubated +/- ET (300 ng/mL). The resultant macrophage supernatant was added to cultures of target marrow granulocyte-macrophage progenitor cells (GM-CFC) at a volume of 1:10. The GM-CFC growth as a percentage of cultures not containing macrophage supernatant were compared and reductions in the number of GM-CFC taken as an index of marrow suppression, Macrophages obtained from B and B + I animals reduced target GM-CFC growth, compared with macrophages from normal animals (B vs, normal animals p < 0.05). In addition, ET-stimulated macrophages induced further bone marrow suppression for all three groups (p < 0.01). Macrophages from granulocyte colony-stimulating factor-treated animals caused significantly less bone marrow suppression, compared with untreated animals for all groups (p < 0.05 to 0.01). Granulocyte colony-stimulating factor administration postburn, in addition to serving as a direct bone marrow stimulant, also alters the macrophage secretory profile and ameliorates macrophage-induced bone marrow suppression.