Spatial variability pattern of the anaerobic ammonia-oxidizing bacterial community across a salinity gradient from river to ocean
Spatial variability pattern of the anaerobic ammonia-oxidizing bacterial community across a salinity gradient from river to ocean
复制标题
从河流到海洋的盐度梯度厌氧氨氧化细菌群落的空间变异模式
DOI：
10.1007/s10646-020-02282-5
复制
发表时间：
2021
期刊：
影响因子：
2.7
通讯作者：
Ye Fei
中科院分区：
文献类型：
--
作者：
Li Yiben;Hong Yiguo;Wu Jiapeng;Wang Yu;Ye Fei
在自然生境中，厌氧氨氧化细菌的多样性会受到多种环境变量的影响。本文研究了从东江（河流沉积物，DJ）到珠江口（河口沉积物，PRE）再到南海（近岸沉积物，SCS）的表层沉积物中厌氧氨氧化细菌群落的分布。结果表明，三种生境中厌氧氨氧化细菌的结构多样性存在明显差异，DJ生境中以Brocadia为优势种，约占总厌氧氨氧化细菌的90%，而Ca.斯卡利杜亚在南海占主导地位。然而，Ca。加利福尼亚州斯卡林杜瓦Brocadia和Ca.在PRE中，Kuenenia共存。qPCR结果显示，厌氧氨氧化细菌16 S rRNA基因丰度范围为2.23 × 105 ~ 1.19 × 107 copies g− 1湿重，但丰度与环境变量之间没有显著相关性（p> 0.05）。Ca.随着盐度的增加，Brocadia逐渐减少，Ca. Scalindua则表现出相反的趋势，表明盐度是塑造自然环境中厌氧氨氧化菌群落组成的关键因素。布罗卡迪亚应该能够生活在淡水生态系统中，但它也可以忍受一定程度的盐度。Scalindua是一种嗜盐厌氧氨氧化细菌，仅存在于盐环境中。根据我们的研究，Kuenenia可以适应广泛的盐度，并喜欢生活在高DIN水平的条件下。厌氧氨氧化菌的分布格局可能是微生物迁移和长期适应盐度的结果。
In natural habitats, the diversity of anaerobic ammonia-oxidizing (anammox) bacteria could be affected by multiple environmental variables. In this study, we investigated the distribution of the anammox bacterial community in surface sediment from the Dongjiang River (riverine sediment, DJ) to the Pearl River Estuary (estuarine sediment, PRE) and then to the South China Sea (coastal sediment, SCS). The results revealed evident differences in the structural diversity of anammox bacteria in three different habitats.CandidatusBrocadia accounted for approximately 90% of the total anammox bacteria in DJ, conversely,Ca. Scalindua dominated in the SCS. Nevertheless,Ca. Scalindua,Ca. Brocadia andCa. Kuenenia coexisted in the PRE. The qPCR results indicated that anammox bacterial 16S rRNA gene abundance ranged from 2.23 × 105to 1.19 × 107copies g−1of wet weight, but no significant correlation was found between the abundances and environmental variables (p> 0.05). The relative abundances ofCa. Brocadia gradually decreased with increasing salinity, andCa. Scalindua showed the opposite trend, suggesting that salinity was a crucial factor in sculpturing the community composition of anammox bacteria in natural environments.Ca. Brocadia should be able to live in freshwater ecosystems, but it can also tolerate a certain level of salinity.Ca. Scalindua was halophilic anammox bacterium and exists only in saline environments.Ca. Kuenenia could adapt to a wide range of salinity and preferred to live in high DIN level conditions according to our search. The distribution pattern of anammox bacteria may be the result of microbial migration and long-term adaptation to salinity.