Electroacupuncture-modulated extracellular ATP levels in prefrontal cortex ameliorated depressive-like behavior of maternal separation rats
Electroacupuncture-modulated extracellular ATP levels in prefrontal cortex ameliorated depressive-like behavior of maternal separation rats
复制标题
DOI：
10.1016/j.bbr.2023.114548
复制
发表时间：
2023-06
影响因子：
2.7
通讯作者：
Yuanjia Zheng;Lingyun Pan;Jiang He;Jinglan Yan;Yucen Xia;Chuqi Lin;Xiuyun Chen;Qianyi Zhao
中科院分区：
文献类型：
--
作者：
Yuanjia Zheng;Lingyun Pan;Jiang He;Jinglan Yan;Yucen Xia;Chuqi Lin;Xiuyun Chen;Qianyi Zhao
产妇分离（MS）是一种早期生活压力，与神经精神疾病，尤其是抑郁症有关。越来越多的证据表明，前额皮质 (PFC) 中的三磷酸腺苷 (ATP) 水平与抑郁症的病理生理学有关。为了研究 PFC 中的 ATP 与电针 (EA) 治疗的抗抑郁作用之间的潜在关系，我们评估了参与 ATP 生物合成的基因以及暴露于新生儿 MS 的大鼠模型中的细胞外 ATP 水平。我们的结果表明，暴露于 MS 的抑郁样大鼠 PFC 中 ABCG2（一种 ATP 结合盒蛋白）表达和 ATP 水平的降低可以通过电针刺激百会 (GV20) 和印堂 (GV29) 穴位来减弱。此外，使用苏拉明（一种广泛的嘌呤能 P2 受体拮抗剂）可以阻断 EA 治疗的抗抑郁作用。总之，这些结果表明电针可能能够调节抑郁样 MS 大鼠 PFC 中的细胞外 ATP 水平，从而可能有助于其抗抑郁作用。
Maternal separation (MS) is a type of early-life stress that has been linked to neuropsychiatric disorders, especially depression. Increasing evidence indicates that the adenosine triphosphate (ATP) level in the prefrontal cortex (PFC) is involved in the pathophysiology of depression. To investigate the potential relationship between ATP in PFC and antidepressant effects of electroacupuncture (EA) treatment, we assessed genes involved in ATP biosynthesis as well as the extracellular ATP levels in a rat model exposed to neonatal MS. Our results demonstrated that reduced expression of ABCG2 (an ATP-binding cassette protein) and ATP levels in the PFC of depressive-like rats exposed to MS can be attenuated by EA stimulus at the Baihui (GV20) and Yintang (GV29) acupoints. Moreover, the antidepressant effect of EA treatment was blocked by administration of suramin, a broad purinergic P2 receptor antagonist. Together, these results suggested that electroacupuncture may be able to modulate extracellular ATP levels in the PFC of depressive-like MS rats, potentially contributing to its antidepressant effects.