Scion genotypes exert long distance control over rootstock transcriptome responses to low phosphate in grafted grapevine
Scion genotypes exert long distance control over rootstock transcriptome responses to low phosphate in grafted grapevine
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DOI：
10.1186/s12870-020-02578-y
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发表时间：
2020-08-03
影响因子：
5.3
通讯作者：
Cookson, Sarah J.
中科院分区：
文献类型：
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作者：
Gautier, Antoine T.;Cochetel, Noe;Cookson, Sarah J.
背景嫁接广泛应用于园艺学中，并且已知砧木可以改变接穗生长和对土壤条件的适应。然而，接穗基因型在调节砧木发育和功能方面的作用在很大程度上仍未被探索。在这项研究中，两个葡萄藤型的相互嫁接，以及相应的同源嫁接控制。这些植物进行了低磷酸盐（LP）治疗和转录组分析RNA测序进行根样品收集后27小时的LP treatment.ResultsA组的转录响应LP治疗在所有接穗/砧木组合进行了鉴定。通过比较两种同种移植物的反应，鉴定了与响应LP的遗传变异相关的基因表达模式。此外，接穗被证明以砧木依赖性方式修改根转录组对LP的响应。一个加权的基因共表达网络分析确定模块的相关基因;这些模块与磷酸盐处理的关联分析，和接穗和砧木基因型确定潜在的枢纽genes.ConclusionsThis研究提供了深入了解嫁接葡萄藤对磷酸盐供应的反应，并确定潜在的芽到根的信号，可以不同葡萄藤基因型之间的变化。
BackgroundGrafting is widely used in horticulture and rootstocks are known to modify scion growth and adaptation to soil conditions. However, the role of scion genotype in regulating rootstock development and functioning has remained largely unexplored. In this study, reciprocal grafts of two grapevine genotypes were produced as well as the corresponding homo-graft controls. These plants were subjected to a low phosphate (LP) treatment and transcriptome profiling by RNA sequencing was done on root samples collected 27h after the onset of the LP treatment.ResultsA set of transcripts responsive to the LP treatment in all scion/rootstock combinations was identified. Gene expression patterns associated with genetic variation in response to LP were identified by comparing the response of the two homo-grafts. In addition, the scion was shown to modify root transcriptome responses to LP in a rootstock dependent manner. A weighted gene co-expression network analysis identified modules of correlated genes; the analysis of the association of these modules with the phosphate treatment, and the scion and rootstock genotype identified potential hub genes.ConclusionsThis study provides insights into the response of grafted grapevine to phosphate supply and identifies potential shoot-to-root signals that could vary between different grapevine genotypes.