Profiling of bacterial flora in gastric biopsies from patients with Helicobacter pylori-associated gastritis and histologically normal control individuals by temperature gradient gel electrophoresis and 16S rDNA sequence analysis
Profiling of bacterial flora in gastric biopsies from patients with Helicobacter pylori-associated gastritis and histologically normal control individuals by temperature gradient gel electrophoresis and 16S rDNA sequence analysis
复制标题
DOI：
10.1099/0022-1317-49-9-817
复制
发表时间：
2000-09-01
影响因子：
3
通讯作者：
Jonasson, J
中科院分区：
文献类型：
--
作者：
Monstein, HJ;Tiveljung, A;Jonasson, J
本研究的目的是通过pcr扩增的16S rDNA片段的时间温度梯度凝胶电泳（TTGE）建立幽门螺杆菌相关性胃炎患者胃活检标本中的细菌谱。来自8名无症状胃炎患者和5名组织学正常对照的标本显示，在大多数胃炎患者的活检中，TTGE谱中存在幽门螺杆菌特异性DNA带，且幽门螺杆菌特异性DNA量增加。胃中还发现了其他属的DNA，包括肠球菌、假单胞菌、链球菌、葡萄球菌和口球菌。在没有胃炎的情况下，幽门螺杆菌似乎是胃活检标本中发现的一个复杂的、可能是本地微生物菌群的一部分。
The aim of this study was to establish bacterial profiles in gastric biopsy specimens from patients with Helicobacter pylori-associated gastritis by means of temporal temperature gradient gel electrophoresis (TTGE) of PCR-amplified 16S rDNA fragments. Specimens from eight patients with asymptomatic gastritis and five histologically normal controls revealed a Helicobacter-specific band in the TTGE profile with increased amounts of Helicobacter-specific DNA in the biopsies from most of the gastritis patients. DNA from other genera Including Enterococcus, Pseudomonas, Streptococcus, Staphylococcus and Stomatococcus was also found in the stomach. In the absence of gastric inflammation, Helicobacter spp, appeared to be part of a complex, presumably indigenous microbial flora found in the biopsy specimens from the stomach.