Leptospiral outer membrane protein LMB216 is involved in enhancement of phagocytic uptake by macrophages
Leptospiral outer membrane protein LMB216 is involved in enhancement of phagocytic uptake by macrophages
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DOI：
10.1111/cmi.12296
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发表时间：
2014-09
影响因子：
3.4
通讯作者：
C. Toma;Gerald Murray;Toshitsugu Nohara;M. Mizuyama;N. Koizumi;B. Adler;Toshihiko Suzuki
中科院分区：
文献类型：
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作者：
C. Toma;Gerald Murray;Toshitsugu Nohara;M. Mizuyama;N. Koizumi;B. Adler;Toshihiko Suzuki
问号钩端螺旋体是引起人畜共患病钩端螺旋体病的病原体。这种螺旋体的致病机制仍然知之甚少，然而，毒力与巨噬细胞内吞噬吸收和存活增加相关。钩端螺旋体外膜蛋白被认为是负责持久性在体内通过与特定的主机组件的相互作用。在这项研究中，我们分析了强毒株及其培养减毒衍生菌株的转录谱，以确定可能参与发病机制的细菌因子。两种外膜蛋白LMB 216和LigB（钩端螺旋体免疫球蛋白样蛋白B）在培养减毒株中下调超过10倍。我们发现，这两种蛋白质在巨噬细胞摄取钩端螺旋体中发挥作用，LMB216以及LigB增强了钩端螺旋体与纤连蛋白的结合。综上所述，我们的研究结果表明LMB216在病原体与宿主分子的相互作用中起作用，这可能有助于钩端螺旋体病的发病机制。
Leptospira interrogans is responsible for the zoonotic disease leptospirosis. The pathogenic mechanisms of this spirochaete remain poorly understood; however, virulence has been correlated with increased phagocytic uptake and survival within macrophages. Leptospiral outer membrane proteins are thought to be responsible for persistence in vivo via interaction with specific host components. In this study, we analysed the transcriptional profile of a virulent strain and its culture‐attenuated derivative strain to identify bacterial factors that may be involved in pathogenesis. Two outer membrane proteins, LMB216 and LigB (leptospiral immunoglobulin‐like protein B) were downregulated more than 10‐fold in the culture‐attenuated strain. We show that both proteins play a role in leptospiral uptake by macrophages and that LMB216, as well as LigB, enhances the binding of leptospires to fibronectin. Taken together, our results indicate that LMB216 plays a role in pathogen interaction with host molecule/s, which may contribute to pathogenesis of leptospirosis.