Enterocyte expression of interleukin 7 induces development of gammadelta T cells and Peyer's patches.
Enterocyte expression of interleukin 7 induces development of gammadelta T cells and Peyer's patches.
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白介素7的肠细胞表达诱导了伽玛达尔塔T细胞和佩耶的斑块的发展。
DOI：
10.1084/jem.191.9.1569
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发表时间：
2000-05-01
影响因子：
15.3
通讯作者：
Puddington, L
中科院分区：
文献类型：
--
作者：
Laky, K;Lefrancois, L;Lingenheld, E G;Ishikawa, H;Lewis, J M;Olson, S;Suzuki, K;Tigelaar, R E;Puddington, L
肠粘膜支持胸腺外T细胞发育，特别是T细胞受体（TCR）-γδ上皮内淋巴细胞（IEL）。TCR-γδ细胞的发育需要白细胞介素（IL）-7; IL-7−/−或IL-7受体−/−小鼠缺乏TCR-γδ细胞。使用肠脂肪酸结合蛋白（iFABP）启动子，我们恢复了IL-7在IL-7−/−小鼠（iFABP-IL 7）成熟肠细胞中的表达。在iFABP-1 L7小鼠中，TCR-γδ IEL恢复，如同隐斑和派伊尔斑一样。TCR-γδ细胞在所有其他组织中保持不存在。同样，胸腺中的T细胞发育以及骨髓和脾脏中的B细胞成熟保留了IL-7−/−表型。因此，肠上皮细胞的IL-7表达足以使TCR-γδ细胞在肠上皮内原位胸腺外发育，并且对于粘膜淋巴组织的组织化至关重要。
The intestinal mucosa is suggested to support extrathymic T cell development, particularly for T cell receptor (TCR)-γδ intraepithelial lymphocytes (IELs). TCR-γδ cell development requires interleukin (IL)-7; IL-7−/− or IL-7 receptor−/− mice lack TCR-γδ cells. Using the intestinal fatty acid binding protein (iFABP) promoter, we reinstated expression of IL-7 to mature enterocytes of IL-7−/− mice (iFABP-IL7). In iFABP-IL7 mice, TCR-γδ IELs were restored, as were cryptopatches and Peyer's patches. TCR-γδ cells remained absent from all other tissues. Likewise, T cell development in thymus and B cell maturation in the bone marrow and spleen retained the IL-7−/− phenotype. Thus, IL-7 expression by enterocytes was sufficient for extrathymic development of TCR-γδ cells in situ within the intestinal epithelium and was crucial for organization of mucosal lymphoid tissue.