Potential use of the Pteris vittata arsenic hyperaccumulation-regulation network for phytoremediation
Potential use of the Pteris vittata arsenic hyperaccumulation-regulation network for phytoremediation
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Pteris vittata砷超积累调节网络在植物修复中的潜在用途
DOI：
10.1016/j.jhazmat.2019.01.072
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发表时间：
2019
影响因子：
13.6
通讯作者：
He Zhenyan
中科院分区：
文献类型：
--
作者：
Yan Huili;Gao Yiwei;Wu Lulu;Wang Luyao;Zhang Tian;Dai Changhua;Xu Wenxiu;Feng Lu;Ma Mi;Zhu Yong-Guan;He Zhenyan
土壤中砷的积累是一个全球性的问题，通常采用植物修复的方法来解决，模式砷超积累植物-蜈蚣蕨作为基因工程植物具有很大的潜力。然而，由于缺乏该物种的组学信息，严重限制了其砷超积累和调节组分的鉴定和应用。在这项研究中，我们使用了一个优化的单分子实时（SMRT）的策略来创建一个新的全长转录组液泡膜蛋白质组数据库，这种未测序的蕨类植物，并确定其砷超积累调控机制的遗传成分。我们建立了一个全面的网络，包括六个主要的转运蛋白家族，两个新的耐药途径，和一个监管系统，通过检查选择性剪接（AS）和长链非编码RNA（lncRNA）在不同的组织后As（III）和As（V）的治疗。本研究所建立的数据库和网络将加深我们对P独特的超积累和调控机制的理解。为进一步研究砷污染土壤的植物修复提供了宝贵的资源。
Arsenic accumulation in soil is a global problem typically addressed using phytoremediation methods.Pteris vittata, a model arsenic hyperaccumulator, has great potential as a genetically engineered plant for phytoremediation. However, the lack of omic information on this species has severely limited the identification and application of its arsenic hyperaccumulation and regulation components. In this study, we used an optimized single-molecular real-time (SMRT) strategy to create ade novofull-length transcriptomic–tonoplast proteomic database for this unsequenced fern and to determine the genetic components underlying its arsenic hyperaccumulation-regulation mechanisms. We established a comprehensive network consisting of six major transporter families, two novel resistance pathways, and a regulatory system by examining alternative splicing (AS) and long non-coding RNA (lncRNA) in different tissues following As(III) and As(V) treatment. The database and network established in this study will deepen our understanding of the unique hyperaccumulation and regulation mechanisms ofP. vittata, ultimately providing a valuable resource for futher research on phytoremediation of arsenic-contaminated soil.