Genetic studies of influenza viruses. I. Viral morphology and growth capacity as exchangeable genetic traits. Rapid in ovo adaptation of early passage Asian strain isolates by combination with PR8.
Genetic studies of influenza viruses. I. Viral morphology and growth capacity as exchangeable genetic traits. Rapid in ovo adaptation of early passage Asian strain isolates by combination with PR8.
复制标题
流感病毒的遗传研究。 I.作为可交换遗传特征的病毒形态和生长能力。通过与Pr8结合使用的早期通过亚洲菌株分离株的OVO适应快速。
DOI：
10.1084/jem.111.3.387
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发表时间：
1960-03-01
影响因子：
15.3
通讯作者：
MURPHY, J S
中科院分区：
文献类型：
--
作者：
KILBOURNE, E D;MURPHY, J S
新分离的丝状亚洲（A2）流感病毒在非感染性PR8（A）病毒存在下的传代导致具有亚洲（A2）抗原性但具有PRS样生长能力和球形形态的病毒快速出现。有证据表明，这种影响的结果从感染性亚洲和非感染性PR8病毒的遗传相互作用，而不是从亚洲株的自发变化。它的结论是，流感病毒的形态，生长速度和生长能力相关联的遗传性状，区分适应适应菌株，并通过重组转移。这些实验的一个实际结果是，已经确定了早期传代流感病毒分离株快速适应鸡胚尿囊的条件。这种适应伴随着病毒产量的增加，这对未来具有新抗原类型的流行病期间的疫苗生产具有明显的意义。
The passage of newly isolated, filamentous Asian (A2) influenza viruses in the presence of non-infective PR8 (A) virus results in the rapid emergence of virus of Asian (A2) antigenicity but PRS-like growth capacity and spherical morphology. Evidence is presented that this effect results from genetic interaction of the infective Asian and non-infective PR8 viruses rather than from spontaneous change of the Asian strain. It is concluded that influenza viral morphology, growth rate and growth capacity are associated genetic traits which distinguish unadapted from adapted strains, and which are transferable by recombination. A pragmatic consequence of these experiments is the fact that conditions have been defined for the rapid adaptation of early passage influenza virus isolates to the chick embryo allantoic sac. Such adaptation is attended by an increase in viral yield which has obvious implications for vaccine production during future epidemics with new antigenic types.