Cross-regulation of biphenyl- and salicylate-catabolic genes by two regulatory systems in Pseudomonas pseudoalcaligenes KF707
Cross-regulation of biphenyl- and salicylate-catabolic genes by two regulatory systems in Pseudomonas pseudoalcaligenes KF707
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DOI：
10.1128/jb.00329-06
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发表时间：
2006-07-01
影响因子：
3.2
通讯作者：
Furukawa, Kensuke
中科院分区：
文献类型：
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作者：
Fujihara, Hidehiko;Yoshida, Hideyuki;Furukawa, Kensuke
假产碱假单胞菌 KF707 以联苯和水杨酸盐为唯一碳源生长。联苯分解代谢 (bph) 基因被组织为 bphR1A1A2(orf3)A3A4BCX0X1X2X3D，编码将联苯转化为乙酰辅酶 A 的酶。在这项研究中，编码将水杨酸转化为乙酰辅酶 A 的酶的水杨酸分解代谢 (sal) 基因簇在 bph 基因簇下游 6.6 kb 处被鉴定，以及第二个基因簇。调控基因，bphR2。在存在或不存在效应子的情况下，bph 和 sal 基因均受到两种调节蛋白 BphR1 和 BphR2 的正向和/或负向交叉调节。 BphR2 结合序列与多种萘降解细菌中的 NahR 结合序列表现出同源性。基于之前的研究和本研究，我们提出了菌株 KF707 中联苯和水杨酸分解代谢的新调控模型。
Pseudomonas pseudoalcaligenes KF707 grows on biphenyl and salicylate as sole sources of carbon. The biphenyl-catabolic (bph) genes are organized as bphR1A1A2(orf3)A3A4BCX0X1X2X3D, encoding the enzymes for conversion of biphenyl to acetyl coenzyme A. In this study, the salicylate-catabolic (sal) gene cluster encoding the enzymes for conversion of salicylate to acetyl coenzyme A were identified 6.6-kb downstream of the bph gene cluster along with a second regulatory gene, bphR2. Both the bph and sal genes were cross-regulated positively and/or negatively by the two regulatory proteins, BphR1 and BphR2, in the presence or absence of the effectors. The BphR2 binding sequence exhibits homology with the NahR binding sequences in various naphthalene-degrading bacteria. Based on previous studies and the present study we propose a new regulatory model for biphenyl and salicylate catabolism in strain KF707.