INDUCTION OF FOS IMMUNOREACTIVITY IN THE RAT-BRAIN AFTER COLD-RESTRAINT INDUCED GASTRIC-LESIONS AND FECAL EXCRETION
INDUCTION OF FOS IMMUNOREACTIVITY IN THE RAT-BRAIN AFTER COLD-RESTRAINT INDUCED GASTRIC-LESIONS AND FECAL EXCRETION
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DOI：
10.1016/0006-8993(94)90316-6
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发表时间：
1994-07-25
期刊：
影响因子：
2.9
通讯作者：
TACHE, Y
中科院分区：
文献类型：
--
作者：
BONAZ, B;TACHE, Y
冷束缚通过迷走神经改变胃肠功能。采用免疫组织化学Fos-IR法，对单独饲养于半圆柱形束缚笼中的禁食大鼠脑内冷暴露3h后激活的神经元通路进行定位。还监测了胃部病变和粪便小球排出量。冷(4℃)束缚3h后，在迷走神经背侧运动核、中缝苍白核、蓝斑和下丘脑室旁核内可见大量Fos阳性核，在中缝隐窝、锥体旁区、延髓去甲肾上腺素能区、终纹床核和隔区也可见少量Fos阳性核。粪便小球产量增加8倍，胃粘膜病变占胃粘膜的19.5+/-1.1%。在其他条件相同的情况下，在室温下束缚的大鼠，这些脑核内Fos阳性细胞很少或没有，没有胃侵蚀，颗粒产量低(1.3+/-：0.5nb/3h)。除了以前的功能研究，这些数据还为中缝尾侧核、迷走神经背侧运动核和下丘脑室旁核神经元参与冷束缚的自主神经和内分泌反应提供了解剖学支持。
Cold-restraint alters gastrointestinal function through vagal pathways. Immunohistochemical detection of the nuclear phosphoprotein Fos (Fos-IR) was used to map brain neuronal pathways activated by cold exposure for 3 h in fasted rats maintained individually in semi-cylindrical restraining cages. Gastric lesions and fecal pellet output were also monitored. In rats exposed to cold (4 degrees C) restraint for 3 h, numerous Fos-positive nuclei were observed in the dorsal motor nucleus of the vagus, raphe pallidus, locus coeruleus, and paraventricular nucleus of the hypothalamus, and, to a lesser extent, in the raphe obscurus, parapyramidal region, and medullary noradrenergic region, bed nucleus of the stria terminalis and septum. Fecal pellet output was increased by 8 fold and gastric lesions covered 19.5 +/- 1.1% of the corpus mucosa. Rats restrained at room temperature under otherwise same conditions had little or no Fos-positive cells in these brain nuclei, no gastric erosion and a low pellet output (1.3 +/-: 0.5 nb/3 h). These data, in addition to previous functional studies, provide anatomic support for the involvement of neurons in the caudal raphe nuclei, dorsal motor nucleus of the vagus and paraventricular nucleus of the hypothalamus in the autonomic and endocrine responses to cold-restraint.