iTRAQ-based proteomic analysis identifies proteins involved in limb regeneration of swimming crab Portunus tritubercuLatus
iTRAQ-based proteomic analysis identifies proteins involved in limb regeneration of swimming crab Portunus tritubercuLatus
复制标题
基于 iTRAQ 的蛋白质组学分析鉴定了参与三疣梭子蟹肢体再生的蛋白质
DOI：
10.1016/j.cbd.2018.02.003
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发表时间：
2018-06-01
影响因子：
3
通讯作者：
Wang, Chunlin
中科院分区：
文献类型：
--
作者：
Fu, Yuanyuan;Zhu, Fang;Wang, Chunlin
梭子蟹（Portunus tntuberculoatm）具有惊人的肢体再生能力，近年来引起了许多研究者的兴趣，本研究采用同量异位素标记相对和绝对定量（iTRAQ）方法研究梭子蟹肢体再生过程中蛋白质丰度的变化。共鉴定了1830个蛋白质，其中181个显著差异表达，94个上调，87个下调。我们的研究结果强调了肢体再生的复杂性及其通过各种生物过程的合作进行的调节，包括细胞骨架变化，细胞外基质（ECM）重塑和ECM-受体相互作用，蛋白质合成，信号识别和转导，能量产生和转换，以及物质运输和代谢。此外，实时PCR证实差异表达基因的mRNA水平与蛋白水平相关。我们的研究结果为研究蟹肢再生的调控机制提供了基础。
The swimming crab (Portunus tntuberculatm) has a striking capacity for limb regeneration, which has drawn the interest of many researchers.In this study, isobaric tag for relative and absolute quantitation (iTRAQ) approach was utilised to investigate protein abundance changes during limb regeneration in this species. A total of 1830 proteins were identified, of which 181 were significantly differentially expressed, with 94 upregulated and 87 downregulated. Our results highlight the complexity of limb regeneration and its regulation through cooperation of various biological processes including cytoskeletal changes, extracellular matrix (ECM) remodelling and ECM- receptor interactions, protein synthesis, signal recognition and transduction, energy production and conversion, and substance transport and metabolism. Additionally, real-time PCR confirmed that mRNA levels of differentially expressed genes were correlated with protein levels. Our results provide a basis for studying the regulatory mechanisms associated with crab limb regeneration.