An aromatic hydroxylation assay for hydroxyl radicals utilizing high-performance liquid chromatography (HPLC). Use to investigate the effect of EDTA on the Fenton reaction.
An aromatic hydroxylation assay for hydroxyl radicals utilizing high-performance liquid chromatography (HPLC). Use to investigate the effect of EDTA on the Fenton reaction.
复制标题
DOI：
10.3109/10715768609051634
复制
发表时间：
1986-01-01
期刊：
影响因子：
--
通讯作者：
Halliwell, B
中科院分区：
文献类型：
--
作者：
Grootveld, M;Halliwell, B
建立了一种高灵敏度的高效液相色谱法，用于分离羟基自由基与苯酚反应生成的羟基化产物。邻苯二酚和对苯二酚是主要的羟基化产物，间苯二酚含量很少。EDTA对铁（II）-H2 O2体系产生羟基自由基的影响取决于螯合剂和金属离子加入反应混合物的顺序、[铁盐]/[螯合剂]的比例以及磷酸盐缓冲液的存在或不存在。这些不同的影响的原因进行了讨论。
A highly sensitive HPLC method for the separation of hydroxylation products derived from the attack of hydroxyl radical upon phenol is described. Catechol and hydroquinone are the major hydroxylation products formed, with little resorcinol. The effect of EDTA upon hydroxyl radical generation from an iron (II)-H2O2 system is shown to depend upon the order of addition of chelator and metal ion to the reaction mixture, the ratio [iron salt]/[chelator] and the presence or absence of a phosphate buffer. Reasons for these different effects are discussed.