Estrogen receptor analysis on biopsies and fine-needle aspirates from human breast carcinoma. Correlation of biochemical and immunohistochemical methods using monoclonal antireceptor antibodies.
Estrogen receptor analysis on biopsies and fine-needle aspirates from human breast carcinoma. Correlation of biochemical and immunohistochemical methods using monoclonal antireceptor antibodies.
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发表时间：
1986-12
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通讯作者：
A. Reiner;J. Spona;G. Reiner;M. Schemper;R. Kolb;W. Kwasny;R. Függer;R. Jakesz;J. Holzner
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作者：
A. Reiner;J. Spona;G. Reiner;M. Schemper;R. Kolb;W. Kwasny;R. Függer;R. Jakesz;J. Holzner
使用针对雌激素受体 (ER) 的单克隆抗体，通过免疫细胞化学方法测定 192 例原发性乳腺癌组织学标本中的 ER 状态。还使用葡聚糖包被木炭法 (DCC) 对所有肿瘤进行 ER 生化分析。生化和免疫细胞化学测定的 ER 状态的比较显示 80% 的结果一致（P 小于 0.0001）。仅 2 例 (1%) ER 水平较低（低于 20 fmol/mg 蛋白质）的免疫细胞化学未能检测到 ER。根据免疫细胞化学染色的强度和异质性，采用半定量方法确定的 ER 阳性与生化测定的 ER 水平显着相关（P = 0.0001）。在 34 例乳腺癌的一系列细针抽吸物中，有足够的细胞材料可用于 ER 免疫细胞化学 (ER-ICA)。总体而言，88% 的样本中细针抽吸物中的 ER-ICA 结果与组织学标本中的 ER-ICA 一致。在少数组织学标本中 ER-ICA 呈弱阳性的病例中，细针抽吸物中 ER-ICA 呈阴性。在肿瘤抽吸物中，免疫细胞化学 ER 测定结果没有出现假阳性。因此，ER-ICA 似乎是一种可靠的检测方法，可以在组织学和细胞学标本中进行。
Monoclonal antibodies against estrogen receptor (ER) were used for determination of ER status immunocytochemically in histologic specimens from 192 primary breast carcinomas. All tumors were also assayed biochemically for ER with the dextran-coated charcoal method (DCC). The comparison of biochemically and immunocytochemically determined ER status showed concordant results in 80% (P less than 0.0001). In only 2 cases (1%) with low ER levels (less than 20 fmol/mg protein) immunocytochemistry failed to detect ER. ER positivity determined with a semiquantified approach based on intensity and heterogeneity of immunocytochemical staining correlated significantly with biochemically determined ER levels (P = 0.0001). In a series of fine-needle aspirates of 34 breast carcinomas sufficient cell material was available for ER immunocytochemistry (ER-ICA). Overall, the results of ER-ICA in fine-needle aspirates were concordant with ER-ICA in histologic specimens in 88% of the samples. In a few cases with weak positivity of ER-ICA in histologic specimens, ER-ICA was negative in fine-needle aspirates. In no case was there a false-positive immunocytochemical ER determination in a tumor aspirate. Thus, ER-ICA seems to be a reliable assay which can be performed in histologic and cytologic specimens.