Evaluation of bone regeneration, angiogenesis, and hydroxyapatite conversion in critical-sized rat calvarial defects implanted with bioactive glass scaffolds
Evaluation of bone regeneration, angiogenesis, and hydroxyapatite conversion in critical-sized rat calvarial defects implanted with bioactive glass scaffolds
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DOI：
10.1002/jbm.a.34272
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发表时间：
2012-12-01
影响因子：
4.9
通讯作者：
Bonewald, Lynda
中科院分区：
文献类型：
--
作者：
Bi, Lianxiang;Jung, Steve;Bonewald, Lynda
生物活性玻璃是生物相容性材料，其在体内转化为羟基磷灰石，并可能支持骨形成，但主要以颗粒形式而不是支架形式获得。在这项研究中，硼硅酸盐和硼酸盐生物活性玻璃支架在临界尺寸的大鼠颅骨缺损进行了评价。将45 S5硅酸盐玻璃颗粒和1393硅酸盐、1393 B1硼硅酸盐和1393 B3硼酸盐玻璃的支架植入15周龄大鼠。12周后，收集缺损，用苏木精和伊红染色以评估骨再生，用高碘酸希夫染色以定量血管面积，用von Kossa和背散射SEM评估新矿化骨和生物活性玻璃的羟基磷灰石转化。45 S5的新骨量为12.4%，1393为8.5%，1393 B1为9.7%，1393 B3为14.9%（*p = 0.04;参见1393和1393 B1）。45 S5（3.8%）的血管面积显著更高（p = 0.009），1393（2.0%）、1393 B1（2.4%）或1393 B3（2.2%）之间无差异。45 S5、1393、1393 B1和1393 B3的von Kossa阳性面积百分比分别为18.7%、25.4%、29.5%和30.1%，1393 B1和1393 B3眼镜中的von Kossa阳性面积百分比显著高于45 S5（p = 0.014）。45 S5和1393 B3在体内完全转化为HA。1393 B3玻璃提供了更大的骨形成，并且由于其能够模塑成支架，可能更有希望用于骨缺损修复。(c)2012 Wiley Periodicals，Inc. J Biomed Mater Res Part A 100 A：32673275，2012.
Bioactive glasses are biocompatible materials that convert to hydroxyapatite in vivo, and potentially support bone formation, but have mainly been available in particulate and not scaffold form. In this study, borosilicate and borate bioactive glass scaffolds were evaluated in critical-sized rat calvarial defects. Twelve-week-old rats were implanted with 45S5 silicate glass particles and scaffolds of 1393 silicate, 1393B1 borosilicate, and 1393B3 borate glass. After 12 weeks, the defects were harvested, stained with hematoxylin and eosin to evaluate bone regeneration, Periodic Acid Schiff to quantitate blood vessel area, and von Kossa and backscatter SEM to estimate newly mineralized bone and hydroxyapatite conversion of bioactive glasses. The amount of new bone was 12.4% for 45S5, 8.5% for 1393, 9.7% for 1393B1, and 14.9% for 1393B3 (*p = 0.04; cf. 1393 and 1393B1). Blood vessel area was significantly higher (p = 0.009) with 45S5 (3.8%), with no differences among 1393 (2.0%), 1393B1 (2.4%), or 1393B3 (2.2%). Percent von Kossa-positive area was 18.7% for 45S5, 25.4% for 1393, 29.5% for 1393B1, and 30.1% for 1393B3, significantly higher (p = 0.014) in 1393B1 and 1393B3 glasses than in 45S5. 45S5 and 1393B3 converted completely to HA in vivo. The 1393B3 glass provided greater bone formation and may be more promising for bone defect repair due to its capacity to be molded into scaffolds. (c) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A 100A:32673275, 2012.