PHENOTYPIC IDENTIFICATION OF AMPLIFICATIONS OF THE ADH4 AND CUP1 GENES OF SACCHAROMYCES-CEREVISIAE
PHENOTYPIC IDENTIFICATION OF AMPLIFICATIONS OF THE ADH4 AND CUP1 GENES OF SACCHAROMYCES-CEREVISIAE
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DOI：
10.1007/bf00312624
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发表时间：
1993-05-01
期刊：
影响因子：
2.5
通讯作者：
PAQUIN, CE
中科院分区：
文献类型：
--
作者：
DORSEY, MJ;HOEH, P;PAQUIN, CE
原代基因扩增，即每个基因组从一个基因拷贝到多个基因拷贝的突变，在基因组进化中是重要的，是产生抗癌耐药的一种手段，并且与肿瘤恶性进展有关。原代扩增尚未在正常真核细胞中进行研究，因为在这些细胞中扩增极为罕见。已经建立了一个系统来表型鉴定酿酒酵母ADH4和CUP1基因的共扩增，并分离出21个独立的自发扩增。
Primary gene amplification, i.e., mutation from one gene copy to multiple gene copies per genome, is important in genomic evolution, as a means of producing anti-cancer drug resistance, and is associated with the progression of tumor malignancy. Primary amplification has not been studied in normal eukaryotic cells because amplifications are extremely rare in these cells. A system has been developed to phenotypically identify co-amplifications of the ADH4 and CUP1 genes of Saccharomyces cerevisiae and 21 independent spontaneous amplifications have been isolated.