Generation and cyclic remodeling of the hair follicle immune system in mice
Generation and cyclic remodeling of the hair follicle immune system in mice
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DOI：
10.1046/j.1523-1747.1998.00243.x
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发表时间：
1998-07-01
影响因子：
6.5
通讯作者：
Hofmann, U
中科院分区：
文献类型：
--
作者：
Paus, R;van der Veen, C;Hofmann, U
在这项免疫组织形态计量学研究中，我们定义了 C57BL/6 小鼠毛囊形态发生和循环过程中毛囊 (HF) 免疫系统与皮肤免疫系统相关的基本特征。朗格汉斯细胞和γδT细胞受体免疫反应性淋巴细胞是新生小鼠皮肤中主要的上皮内造血细胞。在表皮数量增加后，这些细胞迁移到 HF，尽管只有当毛囊形态发生几乎完成时。与朗格汉斯细胞相反，γδT细胞受体免疫反应性淋巴细胞仅通过表皮进入心力衰竭。在整个心力衰竭形态发生和循环过程中，两种细胞类型仍然明显局限于远端外根鞘。在极少数情况下，在HF上皮或皮脂腺内检测到CD4（+）或CD8（+）αβTC，在毛囊周围真皮中的HF发展过程中很早就出现了主要组织相容性复合物II +类，巨噬细胞表型的MAC-1（+）细胞和大量肥大细胞，并且脱颗粒肥大细胞的百分比在HF开始时显着增加。 同步高频循环（第一个退行期），在脱毛和环孢素 A 诱导的高频循环期间，毛囊内朗格汉斯细胞、γδT 细胞受体免疫反应性淋巴细胞和毛囊周围真皮巨噬细胞的数量波动显着。然而，在 HF 消退过程中，未检测到毛囊周围巨噬细胞的数量增加，这对它们在退行期诱导中的作用提出了质疑。总之，HF 免疫系统在毛囊发育过程中相当晚才产生，与毛囊外皮肤免疫系统表现出显着差异，并经历与毛发周期相关的实质性重塑。此外，同步高频循环还伴随着皮肤免疫系统的深刻改变。
In this immunohistomorphometric study, we have defined basic characteristics of the hair follicle (HF) immune system during follicle morphogenesis and cycling in C57BL/6 mice, in relation to the skin immune system. Langerhans cells and gamma delta T cell receptor immunoreactive lymphocytes were the predominant intraepithelial hematopoietic cells in neonatal mouse skin. After their numeric increase in the epidermis, these cells migrated into the HF, although only when follicle morphogenesis was almost completed. In contrast to Langerhans cells, gamma delta T cell receptor immunoreactive lymphocytes entered the HF only via the epidermis, Throughout HF morphogenesis and cycling, both cell types remained strikingly restricted to the distal outer root sheath. On extremely rare occasions, CD4(+) or CD8(+) alpha beta TC were detected within the HF epithelium or the sebaceous gland, Major histocompatibility complex class II+, MAC-1(+) cells of macrophage phenotype and numerous mast cells appeared very early on during HF development in the perifollicular dermis, and the percentage of degranulated mast cells significantly increased during the initiation of synchronized HF cycling (first catagen), During both depilation- and cyclosporine A-induced HF cycling, the numbers of intrafollicular Langerhans cells, gamma delta T cell receptor immunoreactive lymphocytes, and perifollicular dermal macrophages fluctuated significantly. Yet, no numeric increase of perifollicular macrophages was detectable during HF regression, questioning their proposed role in catagen induction. In summary, the HF immune system is generated fairly late during follicle development, shows striking differences to the extrafollicular skin immune system, and undergoes substantial hair cycle-associated remodeling. In addition, synchronized HF cycling is accompanied by profound alterations of the skin immune system.