The effect of the alignment of electrospun fibrous scaffolds on Schwann cell maturation
The effect of the alignment of electrospun fibrous scaffolds on Schwann cell maturation
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DOI：
10.1016/j.biomaterials.2007.10.025
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发表时间：
2008-02-01
期刊：
影响因子：
14
通讯作者：
Leong, Kam W.
中科院分区：
文献类型：
--
作者：
Chew, Sing Ylan;Mi, Ruifa;Leong, Kam W.
在植入前刺激Bungner带的形成可以增强周围神经的再生。制备了排列的电纺聚（ε-己内酯）（PCL）纤维，以测试其向人许旺细胞提供接触引导的潜力。培养7天后，细胞骨架。观察到细胞核沿纤维轴沿着排列和伸长，模拟Bungner带的结构。微阵列分析显示，与接种在二维PCL膜上的细胞相比，对齐细胞中神经营养因子和神经营养受体的表达普遍下调。实时PCR分析证实了早期髓鞘形成标志物髓鞘相关糖蛋白（MAG）的上调和未成熟雪旺细胞标志物NCAM-1的下调。在随机取向的PCL电纺纤维上培养的细胞上也观察到类似的基因表达变化。然而，髓鞘特异性基因PO的上调仅在对齐的电纺纤维上观察到，这表明对齐的纤维在促进雪旺细胞成熟中的倾向。(c)2007爱思唯尔有限公司保留所有权利。
Peripheral nerve regeneration can be enhanced by the stimulation of formation of bands of Bungner prior to implantation. Aligned electrospun poly(epsilon-caprolactone) (PCL) fibers were fabricated to test their potential to provide contact guidance to human Schwann cells. After 7 days of culture, cell cytoskeleton. and nuclei were observed to align and elongate along the fiber axes, emulating the structure of bands of Bungner. Microarray analysis revealed a general down-regulation in expression of neurotrophin and neurotrophic receptors in aligned cells as compared to cells seeded on two-dimensional PCL film. Real-time-PCR analyses confirmed the up-regulation of early myelination marker, myelin-associated glycoprotein (MAG), and the down-regulation of NCAM-1, a marker of immature Schwann cells. Similar gene expression changes were also observed on cells cultured on randomly oriented PCL electrospun fibers. However, upregulation of the myelin-specific gene, PO, was observed only on aligned electrospun fibers, suggesting the propensity of aligned fibers in promoting Schwann cell maturation. (c) 2007 Elsevier Ltd. All rights reserved.