Vitamin E-Labeled Polyethylenimine for in vitro and in vivo Gene Delivery
Vitamin E-Labeled Polyethylenimine for in vitro and in vivo Gene Delivery
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用于体外和体内基因传递的维生素 E 标记聚乙烯亚胺
DOI：
10.1021/acs.biomac.6b00776
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发表时间：
2016-10-01
影响因子：
6.2
通讯作者：
Tang, Xinjing
中科院分区：
文献类型：
--
作者：
Liu, Jinxing;Feng, Mengke;Tang, Xinjing
合成了一系列维生素E（vitE）标记的PEI（PEI-vitE（n）），并与质粒DNA（pDNA）表现出良好的络合能力。PEI-vitE（n）/pDNA-复合物的细胞摄取随着vitE标记的增加而大大增强，在三种不同的细胞系中，这远优于对照PEI 25。PEI-vitE（6）在HEK-293 A细胞中的gfp pDNA递送和随后的GFP表达中显示出最佳性能。此外，在活体小鼠中的体内基因递送也证实了PEI-vitE（6）显示出低毒性，并且有效地将gfp pDNA递送到肝和肺组织的细胞中用于基因表达。
A series of Vitamin E (vitE)-labeled PEIs (PEI-vitE(n)) were synthesized and showed excellent complexation ability with plasmid DNA (pDNA). The cellular uptake of PEI-vitE(n)/pDNA-complexes was greatly enhanced with the increase of vitE labeling, which is much better than that of control PEI25 in three different cell lines. PEI-vitE(6) showed the best performance in gfp pDNA delivery and following GFP expression in HEK-293A cells. In addition, in vivo gene delivery in living mice also confirmed that PEI-vitE(6) showed low toxicity and efficiently delivered gfp pDNA to the cells of liver and lung tissues for gene expression.