Four permeases import proline and the toxic proline analogue azetidine-2-carboxylate into yeast
Four permeases import proline and the toxic proline analogue azetidine-2-carboxylate into yeast
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DOI：
10.1002/yea.1052
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发表时间：
2004-02-01
期刊：
影响因子：
2.6
通讯作者：
Ljungdahl, PO
中科院分区：
文献类型：
--
作者：
Andréasson, C;Neve, EPA;Ljungdahl, PO
我们已经发现，脯氨酸和有毒的脯氨酸类似物氮杂环丁烷-2-羧酸（AzC）被有效地输入到酿酒酵母细胞的四个氨基酸渗透酶，包括两个氮调节的渗透酶（PUT 4和GAP 1）和两个由胞外氨基酸的SPS传感器（AGP 1和GNP 1）调节的渗透酶。与Agp 1 p相反，当细胞在含有脯氨酸作为唯一氮源的培养基上生长时，Gnp 1 p没有功能性表达。这些研究结果的解释使用AzC来表征氮源依赖性调节的氨基酸摄取和后高尔基体的目标和本地化的氨基酸渗透酶在酵母中的研究的影响。版权所有（C）2004约翰威利父子有限公司。
We have found that proline and the toxic proline analogue azetidine-2-carboxylate (AzC) are efficiently imported into Saccharomyces cerevisiae cells by four amino acid permeases, including two nitrogen-regulated permeases (PUT4 and GAP1) and two permeases that are regulated by the SPS sensor of extracellular amino acids (AGP1 and GNP1). In contrast to Agp1p, Gnp1p is not functionally expressed when cells are grown on media containing proline as sole nitrogen source. These findings have implications for the interpretation of studies using AzC to characterize nitrogen source-dependent regulation of amino acid uptake and of post-Golgi targeting and localization of amino acid permeases in yeast. Copyright (C) 2004 John Wiley Sons, Ltd.