Modern Cross-Linking Strategies for Synthesizing Acellular Hemoglobin-Based Oxygen Carriers
Modern Cross-Linking Strategies for Synthesizing Acellular Hemoglobin-Based Oxygen Carriers
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合成基于无细胞血红蛋白的氧载体的现代交联策略
DOI：
10.1002/chin.200935276
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
A. Palmer
中科院分区：
文献类型：
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作者：
D. Harris;A. Palmer
未修饰的无细胞血红蛋白（Hb）在输注到血流中时结构不稳定（Valeri等人，人造细胞血液替代品固定生物技术。2000; 28：451-475; Chan等人，毒理病理学2000; 28：635-642;艾凯，Dissertation，2005;艾凯和Palmer，Biotechnol Prog. 2004; 20：946-952）。本文综述了在过去5年中使用的一些最新的化学策略内和分子间交联血红蛋白，从而稳定其四级结构。因此，本工作将解决以下方面：（1）Hb的位点特异性化学修饰和（2）Hb的非位点特异性化学修饰，包括但不限于PolyHeme VR、Hemopure VR、Oxyglobin VR和SOD-Hb。目前合成聚乙二醇化血红蛋白的策略不在本综述的范围内，本文不再讨论。对于PEG化Hb的更全面的综述，读者可参考以下著作：Cabrales和Friedman，Transfus Alternatives in Transfus Med.2007; 9：281-293和温斯洛，Biochim Biophys Acta，2008; 1784（10）：1382-1386。
Unmodified cell-free hemoglobin (Hb) is structurally unstable when transfused into the blood stream (Valeri et al., Artif Cells Blood Substit Immobil Biotechnol. 2000; 28: 451-475; Chan et al., Toxicol Pathol. 2000; 28: 635-642; Eike, Dissertation, 2005; Eike and Palmer, Biotechnol Prog. 2004; 20: 946-952). This review examines some of the latest chemical strategies used over the last 5 years to intra-and intermolecularly cross-link Hb, thereby stabilizing its quaternary structure. Therefore, this work will address the following aspects:(1) site-specific chemical modifications of Hb and (2) non-site-specific chemical modifications of Hb, including, but not limited to, PolyHeme VR, Hemopure VR, Oxyglobin VR, and SOD-Hb. Current strategies for synthesizing PEGylated Hb is outside the scope of this review and will not be discussed herein. For a more thorough review of PEGylated Hb, the reader is directed to the following works: Cabrales and Friedman, Transfus Alternatives in Transfus Med. 2007; 9: 281–293 and Winslow, Biochim Biophys Acta, 2008; 1784 (10): 1382–1386.