PigS and PigP regulate prodigiosin biosynthesis in Serratia via differential control of divergent operons, which include predicted transporters of sulfur-containing molecules.
PigS and PigP regulate prodigiosin biosynthesis in Serratia via differential control of divergent operons, which include predicted transporters of sulfur-containing molecules.
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DOI：
10.1128/jb.00352-10
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发表时间：
2011-03-01
影响因子：
3.2
通讯作者：
Fineran, Peter C
中科院分区：
文献类型：
--
作者：
Gristwood, Tamzin;McNeil, Matthew B;Fineran, Peter C
沙雷氏菌属菌株ATCC 39006产生红色抗生素灵菌红素。灵菌红素生物合成的调节涉及复杂的层次结构，PigP是参与灵菌红素生产的多个基因的主转录调节因子。本研究的重点是PigP调节子pigS的成员，其编码ArsR/SmtB家族转录抑制子。pigS中的突变通过降低生物合成操纵子的转录来减少灵菌红素的产生。pigS基因是四基因操纵子中的第一个，它还编码COG 2391（DUF 395; YedE/Yeee E）和COG 0730（DUF 81; TauE/SafE）家族的三种膜蛋白（pmpABC），我们建议它们构成含硫化合物的转运组分。我们提供了第一个实验证据，证实了COG 2391蛋白，PmpB的膜定位。从pigS-pmpABC发散地转录的是双顺反子操纵子（blhA-orfY），其编码金属-β-内酰胺酶和含有罗丹酸同源结构域的辅酶A-二硫化物还原酶，这两者都可以参与与含硫化合物的反应。BlhA和OrfY酶和PmpABC膜蛋白的过度生产差异影响色素沉着。我们已经解剖了这些不同的蛋白质的贡献，并确定了它们在灵菌红素生产控制中的重要性。PigS介导的灵菌红素的控制通过直接结合到与pigS和blhA启动子的预测-10区域重叠的基因间区域中的短反向重复序列并抑制转录而发生。PigP是激活这些启动子所必需的，但仅在不存在PigS介导的抑制的情况下。
Serratia sp. strain ATCC 39006 produces the red-pigmented antibiotic prodigiosin. Regulation of prodigiosin biosynthesis involves a complex hierarchy, with PigP a master transcriptional regulator of multiple genes involved in prodigiosin production. The focus of this study was a member of the PigP regulon, pigS, which encodes an ArsR/SmtB family transcriptional repressor. Mutations in pigS reduced production of prodigiosin by decreasing the transcription of the biosynthetic operon. The pigS gene is the first in a four-gene operon, which also encodes three membrane proteins (pmpABC) of the COG2391 (DUF395; YedE/YeeE) and COG0730 (DUF81; TauE/SafE) families that we propose constitute transport components for sulfur-containing compounds. We provide the first experimental evidence confirming the membrane localization of a COG2391 protein, that of PmpB. Divergently transcribed from pigS-pmpABC is a bicistronic operon (blhA-orfY), which encodes a metallo-beta-lactamase and a coenzyme A-disulfide reductase containing a rhodanese homology domain, both of which may participate in reactions with sulfur-containing compounds. The overproduction of the BlhA and OrfY enzymes and the PmpABC membrane proteins differentially affected pigmentation. We have dissected the contributions of these various proteins and determined their importance in the control of prodigiosin production. PigS-mediated control of prodigiosin occurred via binding directly to a short inverted repeat sequence in the intergenic region overlapping the predicted -10 regions of both pigS and blhA promoters and repressing transcription. PigP was required for the activation of these promoters, but only in the absence of PigS-mediated repression.