CD11b regulates recruitment of alveolar macrophages but not pulmonary dendritic cells after pneumococcal challenge
CD11b regulates recruitment of alveolar macrophages but not pulmonary dendritic cells after pneumococcal challenge
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DOI：
10.1086/498874
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发表时间：
2006-01-15
影响因子：
6.4
通讯作者：
Kaye, PM
中科院分区：
文献类型：
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作者：
Kirby, AC;Raynes, JG;Kaye, PM
尽管肺泡巨噬细胞（AM）和肺树突状细胞（pulDC）在物理和功能上关系密切，但很少在感染情况下一起进行检查。使用鼻内注射肺炎链球菌引起的肺炎的非致死性缓解模型，我们证明 AM 和 pulDC 在肺部炎症过程中表现出不同的特征。 AM 和 pulDC 的募集以不同的动力学发生，AM 数量的增加主要是由于 CD11b（高）AM 的独特子集的出现。从支气管肺泡灌洗液中可回收到 CD11bHigh 和 CD11b(Low) AM 数量增加，但 pulDC 数量未增加。粒细胞-巨噬细胞集落刺激因子可显着增加 AM 上的 CD11b 表达，但白细胞介素 10 或病原体相关刺激则不会显着增加 AM 上的 CD11b 表达。最后，抗体阻断证明 CD11b 对于肺炎球菌攻击后将 AM（而非 pulDC）募集到肺部至关重要。这些数据表明AM和pulDC对体内炎症致病刺激的反应存在显着差异。
Despite their close physical and functional relationships, alveolar macrophages (AMs) and pulmonary dendritic cells (pulDCs) have rarely been examined together in the context of infection. Using a nonlethal, resolving model of pneumonia caused by intranasal injection of Streptococcus pneumoniae, we demonstrate that AMs and pulDCs exhibit distinct characteristics during pulmonary inflammation. Recruitment of AMs and pulDCs occurred with different kinetics, and increased numbers of AMs resulted mainly from the appearance of a distinct subset of CD11b(High) AMs. Increased numbers of CD11bHigh and CD11b(Low) AMs, but not pulDCs, were recoverable from bronchoalveolar lavage fluid. CD11b expression on AMs was significantly increased by granulocyte-macrophage colony-stimulating factor but not by interleukin-10 or pathogen-associated stimuli. Finally, antibody blockade demonstrated that CD11b was critical for the recruitment of AMs, but not pulDCs, into the lung after pneumococcal challenge. These data demonstrate that there are significant differences between AM and pulDC responses to inflammatory pathogenic stimuli in vivo.