The STIM-Orai Pathway: The Interactions Between STIM and Orai
The STIM-Orai Pathway: The Interactions Between STIM and Orai
复制标题
DOI：
10.1007/978-3-319-57732-6_4
复制
发表时间：
2017-01-01
期刊：
影响因子：
--
通讯作者：
Romanin, Christoph
中科院分区：
文献类型：
--
作者：
Fahrner, Marc;Schindl, Rainer;Romanin, Christoph
钙释放激活的钙通道是非兴奋性细胞内钙进入的主要途径。它们的两个限制性分子成分包括位于内质网的钙离子感受器蛋白STIM1和质膜上的Orai通道。STIM1感知胞腔内的钙离子含量，存储耗竭导致其寡聚形成点状结构，从而触发与Orai通道的偶联和激活。假设C-末端的STIM1结构域与ORAI的C-末端和N-末端的胞浆链偶联，实现通道的门控。在这里，我们重点介绍了STIM1-Orai信号级联的分子间和分子内步骤，以及实现钙渗透的孔结构的关键位置。
A primary Ca2+ entry pathway in non-excitable cells is established by the Ca2+ release-activated Ca2+ channels. Their two limiting molecular components include the Ca2+-sensor protein STIM1 located in the endoplasmic reticulum and the Orai channel in the plasma membrane. STIM1 senses the luminal Ca (2+) content, and store depletion induces its oligomerization into puncta-like structures, thereby triggering coupling to as well as activation of Orai channels. A C-terminal STIM1 domain is assumed to couple to both C-and N-terminal, cytosolic strands of Orai, accomplishing gating of the channel. Here we highlight the inter-and intramolecular steps of the STIM1-Orai signaling cascade together with critical sites of the pore structure that accomplishes Ca2+ permeation.